Novel anti-neoplastic approach for photodynamic theranostics by biocompatible TiO2 popcorn nanostructure for a high-throughput flash ROS generator
2014 (English)In: 24th Anniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Conference paper, Poster (Refereed)
Reactive oxygen species (ROS) are important secondary messengers in the intracellular signaling system for regulating redox homeostasis in normal cells. Compared to normal cells, cancer cells have increased ROS levels due to a faster metabolic rate. We have used this discriminating overproduction of ROS levels in cancer cells as a target for a photodynamic anti-neoplastic theranostic approach using mesoporous TiO2 microbeads with a popcorn nanostructure. We have created a novel flash ROS generator using a two-step procedure consisting of sol-gel and solvothermal processes to obtain mesoporous TiO2 microbeads with high photocatalytic efficiency. A photon-induced comparative study has been carried out for the ROS generation ability using TiO2 nanoparticles and mesoporous TiO2 microbeads. We have shown that in under otherwise identical conditions the extent of photocatalytical ROS generated by mesoporous TiO2 microbeads is more than twice that produced by TiO2 nanoparticles. In vitro in the absence of irradiation, the mesoporous TiO2 microbeads are exceptionally biocompatible, allowing almost ~100% cellular survival rate even at a dose of 100 µg/mL. In contrast, commercial nanoparticles showed concentration dependent cytotoxicity of nearly 15% within 24h in the absence of any irradiation. Upon photo activation, the mesoporous TiO2 microbead structures delivered their potential anticancer effect by interfering with the mitochondrial activity by producing ROS in the intracellular environment and thus reducing the survival rate of cells by more than 30% in comparison with commercial nanoparticles, where only an increase of 5% in cell death was observed. Thus we have developed a smart on/off switchable photodynamic anti-neoplastic theranostic approach that can be combined with specific cell recognition elements for future cancer management.
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IdentifiersURN: urn:nbn:se:liu:diva-108432OAI: oai:DiVA.org:liu-108432DiVA: diva2:730303
24th Anniversary World Congress on Biosensors – Bios ensors 2014, 27-30 May 2014, Melbourne, Australia