liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Time-resolved botulinum neurotoxin A activity monitored using peptide-functionalized Au nanoparticle energy transfer sensors
Nanyang Technological University, Singapore.
Nanyang Technological University, Singapore.
Nanyang Technological University, Singapore.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, The Institute of Technology. Nanyang Technological University, Singapore.ORCID iD: 0000-0002-7001-9415
Show others and affiliations
2014 (English)In: Chemical Science, ISSN 2041-6520, E-ISSN 2041-6539, Vol. 5, no 7, 2651-2656 p.Article in journal (Refereed) Published
Abstract [en]

We report herein on the employment of synthetic peptide-functionalized gold nanoparticles (AuNPs) with various diameters as radiative quenchers for the time-resolved monitoring of botulinum A light chain (BoLcA) activity. The results demonstrate that larger AuNPs provide higher energy transfer efficiencies between the dye and the AuNPs, but poorer BoLcA activities for the proteolysis of peptides because of steric constraints. The initial turnover number for the BoLcA proteolysis of peptides on 18 nm AuNPs was retarded by a factor of 80 as compared with 1.4 nm AuNPs. A similar phenomenon has been observed for trypsin, however, with less hindrance on large AuNPs. Thus, the use of smaller 1.4 nm AuNPs in conjunction with robust synthetic peptides provides an attractive format for the time-resolved monitoring of protease activity and for BoLcA sensing at a highly competitive limit of detection (1 pM).

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014. Vol. 5, no 7, 2651-2656 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-108945DOI: 10.1039/c3sc53305kISI: 000337108200005OAI: oai:DiVA.org:liu-108945DiVA: diva2:734181
Available from: 2014-07-15 Created: 2014-07-13 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Aili, DanielLiedberg, Bo

Search in DiVA

By author/editor
Aili, DanielLiedberg, Bo
By organisation
Molecular PhysicsThe Institute of Technology
In the same journal
Chemical Science
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 161 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf