A facile "click" reaction to fabricate a FRET-based ratiometric fluorescent Cu2+ probe
2014 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 2, no 28, 4467-4472 p.Article in journal (Refereed) Published
A facile one-step Cu(I)-catalyzed "click" reaction, between a dansyl-azide and a propargyl-substituted rhodamine B hydrazide, is employed to fabricate a novel FRET ratiometric "off-on" fluorescent probe. The sensitive emission of the donor, a dansyl group, overlaps perfectly with the absorption of the acceptor, xanthene in the open-ring rhodamine. The proposed probe shows high selectivity towards Cu2+. The ratio of emission intensities at 568 and 540 nm (I-568/I-540) exhibits a drastic 28-fold enhancement upon addition of Cu2+. The probe shows an excellent linear relationship between emission ratios and the concentrations of Cu2+ from 10 to 50 mu M, with a detection limit (S/N = 3) of 0.12 mu M. The preliminary cellular studies demonstrated that the probe is cell membrane permeable and could be applied for ratiometric fluorescence imaging of intracellular Cu2+ with almost no cytotoxicity. The ingenuity of the probe design is to construct a FRET donor-acceptor interconnector and a selective receptor simultaneously by "click" reaction. The strategy was verified to have great potential for developing novel FRET probes for Cu2+.
Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014. Vol. 2, no 28, 4467-4472 p.
IdentifiersURN: urn:nbn:se:liu:diva-109291DOI: 10.1039/c4tb00441hISI: 000338646000009OAI: oai:DiVA.org:liu-109291DiVA: diva2:737040