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Quantitative evaluation of a single-distance phase-retrieval method applied on in-line phase-contrast images of a mouse lung
Abdus Salam International Centre for Theoretical Physics, Trieste, Italy.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Surface Physics and Nano Science. Linköping University, The Institute of Technology. Synchrotron Light Source `Elettra' Trieste, Italy.
University Hospital Goettingen, Germany .
Cluster Biomed Scrl, Trieste, Italy .
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2014 (English)In: Journal of Synchrotron Radiation, ISSN 0909-0495, E-ISSN 1600-5775, Vol. 21, 784-789 p.Article in journal (Refereed) Published
Abstract [en]

Propagation-based X-ray phase-contrast computed tomography (PBI) has already proven its potential in a great variety of soft-tissue-related applications including lung imaging. However, the strong edge enhancement, caused by the phase effects, often hampers image segmentation and therefore the quantitative analysis of data sets. Here, the benefits of applying single-distance phase retrieval prior to the three-dimensional reconstruction (PhR) are discussed and quantified compared with three-dimensional reconstructions of conventional PBI data sets in terms of contrast-to-noise ratio (CNR) and preservation of image features. The PhR data sets show more than a tenfold higher CNR and only minor blurring of the edges when compared with PBI in a predominately absorption-based set-up. Accordingly, phase retrieval increases the sensitivity and provides more functionality in computed tomography imaging.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2014. Vol. 21, 784-789 p.
Keyword [en]
computed tomography; phase-contrast imaging; phase retrieval; lung imaging
National Category
Physical Sciences
URN: urn:nbn:se:liu:diva-109252DOI: 10.1107/S1600577514009333ISI: 000338124300019PubMedID: 24971975OAI: diva2:737157
Available from: 2014-08-12 Created: 2014-08-11 Last updated: 2015-11-11Bibliographically approved
In thesis
1. Evaluation of the Dual-Modal usage of contrast agents by means of Synchrotron X-ray Computed Microtomography and Magnetic Resonance Imaging using Macrophages loaded with Barium Sulfate and Gadolinium Nanoparticles for Detection and Monitoring in Animal Disease Models
Open this publication in new window or tab >>Evaluation of the Dual-Modal usage of contrast agents by means of Synchrotron X-ray Computed Microtomography and Magnetic Resonance Imaging using Macrophages loaded with Barium Sulfate and Gadolinium Nanoparticles for Detection and Monitoring in Animal Disease Models
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

¨This thesis focuses on evaluating the dual-modal Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) capabilities of contrast agents. For such purposes a gadolinium based contrast agent is of high interest, due to its paramagnetic properties, which while present inside a magnetic field will hence interact with the protons spins of water (in tissue and fat) and shorten their the T1 relaxation time, thereby creating a positive image contrast in MRI. Furthermore, the X-ray Mass Attenuation Coefficient (MAC) of gadolinium is relatively high, thus suggesting its potential use, also as a CT contrast agent.

Gadolinium nanoparticles (GdNPs) can be loaded into cells, such as macrophages, which offers the possibility to track cells inside entire organisms. In the first step the uptake of GdNPs inside cells was investigated, together with a test for toxicity. To show the potential of using GdNP loaded macrophages for functional imaging of inflammation, an acute allergic airway inflammation mouse model (mimicking asthma in humans) was used and analyzed by in-situ synchrotron phase contrast CT. In the first step this approach was evaluated using macrophages loaded with a clinical contrast agent containing barium sulphate (BaSO4), since this agent is known to provide high contrast in CT. In the ultimate step a combination of both BaSO4 and GdNP loaded macrophages was used in the same asthmatic mouse model and analyzed by dual modal Synchrotron phase contrast CT and Micro Magnetic Resonance Imaging (μ-MRI).

Complementary results in terms of the biodistribution of injected macrophages could only be obtained by the combination of both synchrotron phase contrast CT and μ-MRI, where the first modality allows a detailed localization of clustered BaSO4 loaded macrophages, but fails to detect single macrophages, which could instead be indirectly observed by μ-MRI as an increase of the T1-contrast, coming from the soft tissue of mice injected with GdNP loaded macrophages.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2015. 73 p.
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1707
National Category
Physical Sciences Radiology, Nuclear Medicine and Medical Imaging
urn:nbn:se:liu:diva-122607 (URN)10.3384/diss.diva-122607 (DOI)978-91-7685-936-0 (print) (ISBN)
Public defence
2015-12-07, Planck, Fysikhuset, Campus Valla, Linköping, 10:15 (English)
Available from: 2015-11-11 Created: 2015-11-11 Last updated: 2015-12-02Bibliographically approved

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