liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Phosphorylated AKT preserves stallion sperm viability and motility by inhibiting caspases 3 and 7
University of Extremadura, Spain .
University of Extremadura, Spain .
University of Extremadura, Spain .
University of Extremadura, Spain .
Show others and affiliations
2014 (English)In: Reproduction, ISSN 1470-1626, E-ISSN 1476-3990, Vol. 148, no 2, 221-235 p.Article in journal (Refereed) Published
Abstract [en]

AKT, also referred to as protein kinase B (PKB or RAC), plays a critical role in controlling cell survival and apoptosis. To gain insights into the mechanisms regulating sperm survival after ejaculation, the role of AKT was investigated in stallion spermatozoa using a specific inhibitor and a phosphoflow approach. Stallion spermatozoa were washed and incubated in Biggers-Whitten-Whittingham medium, supplemented with 1% polyvinyl alcohol (PVA) in the presence of 0 (vehicle), 10, 20 or 30 mu M SH5, an AKT inhibitor. SH5 treatment reduced the percentage of sperm displaying AKT phosphorylation, with inhibition reaching a maximum after 1 h of incubation. This decrease in phosphorylation was attributable to either dephosphorylation or suppression of the active phosphorylation pathway. Stallion spermatozoa spontaneously dephosphorylated during in vitro incubation, resulting in a lack of a difference in AKT phosphorylation between the SH5-treated sperm and the control after 4 h of incubation. AKT inhibition decreased the proportion of motile spermatozoa (total and progressive) and the sperm velocity. Similarly, AKT inhibition reduced membrane integrity, leading to increased membrane permeability and reduced the mitochondrial membrane potential concomitantly with activation of caspases 3 and 7. However, the percentage of spermatozoa exhibiting oxidative stress, the production of mitochondrial superoxide radicals, DNA oxidation and DNA fragmentation were not affected by AKT inhibition. It is concluded that AKT maintains the membrane integrity of ejaculated stallion spermatozoa, presumably by inhibiting caspases 3 and 7, which prevents the progression of spermatozoa to an incomplete form of apoptosis.

Place, publisher, year, edition, pages
Bioscientifica, 2014. Vol. 148, no 2, 221-235 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:liu:diva-109219DOI: 10.1530/REP-13-0191ISI: 000338540300012PubMedID: 24850868OAI: oai:DiVA.org:liu-109219DiVA: diva2:737203
Available from: 2014-08-12 Created: 2014-08-11 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(1290 kB)421 downloads
File information
File name FULLTEXT01.pdfFile size 1290 kBChecksum SHA-512
0abaef6e44b83378726cc072f0fbc0f6cc21143cbe1d7b10c1162940f835829a96608f7234815eccdf5ea1d4e1025d46bdb4871d8ada671be2c4bbc502db8c05
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Rodriguez-Martinez, Heriberto

Search in DiVA

By author/editor
Rodriguez-Martinez, Heriberto
By organisation
Division of Microbiology and Molecular MedicineFaculty of Health Sciences
In the same journal
Reproduction
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 421 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 137 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf