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A highly UV-transparent fused silica biochip for sensitive hepatotoxicity testing by autofluorescence
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
University of Gothenburg, Sweden .
Lund University, Sweden .
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
2014 (English)In: BioChip Journal, ISSN 1976-0280, Vol. 8, no 2, 115-121 p.Article in journal (Refereed) Published
Abstract [en]

Fabrication and application of a non-fluorescent and UV-transparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture work severely disturb such measurements. In this study the fused silica biochip was used for measuring intrinsic autofluorescence from liver cells in order to assess hepatotoxic effects of drugs. The assessment assay was carried out with the human liver cell line HepG2 under perfusion conditions in the microfluidics of the biochip. The autofluorescence from the.liver cells exposed to quinidine was readily recorded without background disturbance and correlated well with reference toxicity data.

Place, publisher, year, edition, pages
Springer, 2014. Vol. 8, no 2, 115-121 p.
Keyword [en]
Autofluorescence; Drug testing; Microfluidic chip; Fused silica; In vitro toxicity; HepG2 cell line
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:liu:diva-109130DOI: 10.1007/s13206-014-8206-zISI: 000337717900006OAI: oai:DiVA.org:liu-109130DiVA: diva2:737527
Available from: 2014-08-13 Created: 2014-08-11 Last updated: 2017-04-05Bibliographically approved

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Fritzsche, MichaelMandenius, Carl-Fredrik

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  • sv-SE
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