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Structural and Biochemical Characterization of Human PR70 in Isolation and in Complex with the Scaffolding Subunit of Protein Phosphatase 2A
Karolinska Institutet, Stockholm, Sweden.
Lawrence Berkeley National Lab (LBNL), Berkeley, California, USA.
Karolinska Institutet, Stockholm, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology. Karolinska Institutet, Stockholm, Sweden.
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2014 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 7, e0101846- p.Article in journal (Refereed) Published
Abstract [en]

Protein Phosphatase 2A (PP2A) is a major Ser/Thr phosphatase involved in the regulation of various cellular processes. PP2A assembles into diverse trimeric holoenzymes, which consist of a scaffolding (A) subunit, a catalytic (C) subunit and various regulatory (B) subunits. Here we report a 2.0 angstrom crystal structure of the free B /PR70 subunit and a SAXS model of an A/PR70 complex. The crystal structure of B /PR70 reveals a two domain elongated structure with two Ca2+ binding EF-hands. Furthermore, we have characterized the interaction of both binding partner and their calcium dependency using biophysical techniques. Ca2+ biophysical studies with Circular Dichroism showed that the two EF-hands display different affinities to Ca2+. In the absence of the catalytic C-subunit, the scaffolding A-subunit remains highly mobile and flexible even in the presence of the B /PR70 subunit as judged by SAXS. Isothermal Titration Calorimetry studies and SAXS data support that PR70 and the A-subunit have high affinity to each other. This study provides additional knowledge about the structural basis for the function of B containing holoenzymes.

Place, publisher, year, edition, pages
Public Library of Science , 2014. Vol. 9, no 7, e0101846- p.
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Chemical Sciences
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URN: urn:nbn:se:liu:diva-109375DOI: 10.1371/journal.pone.0101846ISI: 000339040600073PubMedID: 25007185OAI: oai:DiVA.org:liu-109375DiVA: diva2:738057
Available from: 2014-08-15 Created: 2014-08-15 Last updated: 2017-12-05Bibliographically approved

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Anandapadmanaban, Madhanagopal

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