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Hydroxymethylated DNA immunoprecipitation (hmeDIP)
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting. MRC Human Genetics Unit, University of Edinburgh, Western General Hospital, Edinburgh, UK.
MRC Human Genetics Unit, University of Edinburgh, Western General Hospital, Edinburgh, UK.
2014 (English)In: Functional analysis of DNA and chromatin / [ed] Juan C Stockert; Jesús Espada; Alfonso Blázquez-Castro, New York: Humana Press, 2014, Vol. 1094, 259-267 p.Chapter in book (Refereed)
Abstract [en]

5-hydroxymethylcytosine (5hmC) was recently identified as an abundant epigenetic mark in mammals. Subsequent research has implicated 5hmC in normal mammalian development and disease pathogenesis in humans. Many of the techniques commonly used to assay for canonical 5-methylcytosine (5mC) cannot distinguish between 5hmC and 5mC. The development of antibodies specific to 5hmC has allowed for specific enrichment of DNA fragments containing 5hmC. Hydroxymethylated DNA immunoprecipitation (hmeDIP) has become an invaluable tool for determining both locus-specific and genome-wide profiles of 5hmC in mammalian DNA. Here, we describe the use of hmeDIP to characterize the relative abundance of 5hmC at loci in mammalian DNA. 

Place, publisher, year, edition, pages
New York: Humana Press, 2014. Vol. 1094, 259-267 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; vol 1094
Keyword [en]
5-Hydroxymethylcytosine; Antibody; DNA; Hydroxymethylation; Immunoprecipitation; Methylation; Sonication
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:liu:diva-110532DOI: 10.1007/978-1-62703-706-8_20ISI: 000332345200021PubMedID: 24162994Scopus ID: 2-s2.0-84891786709ISBN: 978-1-62703-705-1 (print)ISBN: 978-1-62703-706-8 (print)OAI: oai:DiVA.org:liu-110532DiVA: diva2:746848
Note

Funding Agencies|MRC, Medical Research Council; BBSRC, Biotechnology and Biological Sciences Research Council

Available from: 2014-09-15 Created: 2014-09-12 Last updated: 2014-10-09Bibliographically approved

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Nestor, Colm

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