liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Hydroxymethylated DNA immunoprecipitation (hmeDIP)
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting. MRC Human Genetics Unit, University of Edinburgh, Western General Hospital, Edinburgh, UK.
MRC Human Genetics Unit, University of Edinburgh, Western General Hospital, Edinburgh, UK.
2014 (English)In: Functional analysis of DNA and chromatin / [ed] Juan C Stockert; Jesús Espada; Alfonso Blázquez-Castro, New York: Humana Press, 2014, Vol. 1094, 259-267 p.Chapter in book (Refereed)
Abstract [en]

5-hydroxymethylcytosine (5hmC) was recently identified as an abundant epigenetic mark in mammals. Subsequent research has implicated 5hmC in normal mammalian development and disease pathogenesis in humans. Many of the techniques commonly used to assay for canonical 5-methylcytosine (5mC) cannot distinguish between 5hmC and 5mC. The development of antibodies specific to 5hmC has allowed for specific enrichment of DNA fragments containing 5hmC. Hydroxymethylated DNA immunoprecipitation (hmeDIP) has become an invaluable tool for determining both locus-specific and genome-wide profiles of 5hmC in mammalian DNA. Here, we describe the use of hmeDIP to characterize the relative abundance of 5hmC at loci in mammalian DNA. 

Place, publisher, year, edition, pages
New York: Humana Press, 2014. Vol. 1094, 259-267 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; vol 1094
Keyword [en]
5-Hydroxymethylcytosine; Antibody; DNA; Hydroxymethylation; Immunoprecipitation; Methylation; Sonication
National Category
Clinical Medicine
URN: urn:nbn:se:liu:diva-110532DOI: 10.1007/978-1-62703-706-8_20ISI: 000332345200021PubMedID: 24162994ScopusID: 2-s2.0-84891786709ISBN: 978-1-62703-705-1 (print)ISBN: 978-1-62703-706-8 (online)OAI: diva2:746848

Funding Agencies|MRC, Medical Research Council; BBSRC, Biotechnology and Biological Sciences Research Council

Available from: 2014-09-15 Created: 2014-09-12 Last updated: 2014-10-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopusFind book at a Swedish library/Hitta boken i ett svenskt bibliotekFind book in another country/Hitta boken i ett annat land

Search in DiVA

By author/editor
Nestor, Colm
By organisation
Division of Clinical SciencesFaculty of Health SciencesÖstergötlands Läns Landsting
Clinical Medicine

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 40 hits
ReferencesLink to record
Permanent link

Direct link