Overexpression of MMP-9 and Its Inhibitors in Blood Mononuclear Cells after Myocardial Infarction - Is It Associated with Depressive Symptomatology?
2014 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 9, no 8, e105572- p.Article in journal (Refereed) Published
Background: Matrix metalloproteinase (MMP)-9 may play a central role in the development and progression of atherosclerosis. Emerging evidence also indicates an association between MMP-9 and depressive symptomatology. Here, we investigated whether expression of MMP-9 and its inhibitors in blood mononuclear cells and plasma were related to depressive symptoms in patients with a recent myocardial infarction (MI). Methods and Results: Blood sampling was performed between 6 and 18 months after MI in 57 patients. Forty-one clinically healthy subjects were included as controls. Gene expression of MMP-9 and its main tissue inhibitors TIMP-1 and -2 were analyzed in freshly isolated or cultured blood mononuclear cells. Corresponding protein levels were assessed in cell supernatants and plasma. In post-MI patients, mRNA levels of MMP-9 and TIMP-1 and -2 were significantly higher than in controls while protein levels in cell supernatants and plasma did not differ between groups. The Center for Epidemiological Studies - Depression (CES-D) scale was used to assess depressive symptomatology. Repeated assessments during the first 18 months after MI showed significantly higher CES-D scores in patients compared with controls. However, there were no relationships between depressive mood and any of the measurements of MMP-9 or TIMPs. Conclusion: Our findings indicate that overexpression of MMP-9 and TIMPs in blood mononuclear cells and elevated depressive symptoms represent two unrelated phenomena after MI.
Place, publisher, year, edition, pages
Public Library of Science , 2014. Vol. 9, no 8, e105572- p.
IdentifiersURN: urn:nbn:se:liu:diva-110970DOI: 10.1371/journal.pone.0105572ISI: 000340952200062PubMedID: 25153995OAI: oai:DiVA.org:liu-110970DiVA: diva2:752000
Funding Agencies|Swedish Research Council [521-2011-3847]; Swedish Heart-Lung Foundation 2014-10-022014-10-012015-02-18