A rhodamine-based fluorescent probe for Hg2+ and its application for biological visualization
2014 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, Vol. 203, 452-458 p.Article in journal (Refereed) Published
A new visible light excitable fluorescent probe (1) is synthesized by appending a hydroxymethyl-pyridine to rhodamine B hydrazide. The probe displays very specific Hg2+-induced colour change and fluorescent enhancement in the aqueous systems. The "turn-on" response of fluorescence is based on a binding-induced ring-opening process from the spirolactam (nonfluorescent) to acyclic xanthene (fluorescent) in rhodamine B. The coordinating atoms O-center dot-N-N-O-center dot from the hydroxymethyl-pyridine and rhodamine B hydrazide play dominant role in the formation of a complex with 1:1 stoichiometry of Hg2+ to 1. It exhibits a linear response in the range of 0.1-5 mu M with the limit of detection (LOD) of 15.7 nM (3 sigma/slope), while the calculated value of the association constant of Hg2+/1 is 0.70 x 10(5) M-1. Furthermore, confocal microscopy imaging experiment demonstrates the probe 1 can be applied as a fluorescent probe for visualization of Hg2+ in living HeLa cells.
Place, publisher, year, edition, pages
Elsevier , 2014. Vol. 203, 452-458 p.
Mercury; Rhodamine; Fluorescence; Probe; Fluorescent imaging
IdentifiersURN: urn:nbn:se:liu:diva-111253DOI: 10.1016/j.snb.2014.06.104ISI: 000341455400059OAI: oai:DiVA.org:liu-111253DiVA: diva2:755613
Funding Agencies|National Key Technology RandD Program of China [2013BAC01B01]; National Natural Science Foundation of China ; Swedish Government Strategic Faculty Grant in Material Science (SFO, MATLIU) in Advanced Functional Materials [SFO-Mat-LiU#2009-00971]2014-10-152014-10-142014-10-15