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Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Immunology and Transfusion Medicine. Region Östergötland, Center for Diagnostics, Department of Clinical Chemistry.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Chemistry.
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2015 (English)In: Platelets, ISSN 0953-7104, E-ISSN 1369-1635, Vol. 26, no 2, 177-185 p.Article in journal (Refereed) Published
Abstract [en]

The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by aphaeresis technique (n = 7). Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage. Spontaneous and TRAP-6-induced adhesion to fibrinogen- and collagen-coated beads was analyzed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p < 0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p < 0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p < 0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation. We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.

Place, publisher, year, edition, pages
2015. Vol. 26, no 2, 177-185 p.
National Category
Basic Medicine
Identifiers
URN: urn:nbn:se:liu:diva-111533DOI: 10.3109/09537104.2014.891728ISI: 000351740700012PubMedID: 24679340OAI: oai:DiVA.org:liu-111533DiVA: diva2:757422
Available from: 2014-10-22 Created: 2014-10-22 Last updated: 2017-12-05Bibliographically approved

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Tynngård, NahreenWallstedt, MariaSödergren, Anna LFaxälv, LarsRamström, Sofia

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Tynngård, NahreenWallstedt, MariaSödergren, Anna LFaxälv, LarsRamström, Sofia
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Division of Microbiology and Molecular MedicineFaculty of Medicine and Health SciencesDepartment of Clinical Immunology and Transfusion MedicineDepartment of Clinical Chemistry
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