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Den preanalytiska fasen – Hur viktigt är det att blanda serumrören?
Linköping University, Faculty of Health Sciences.
2014 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
The pre-analytical phase – How important is it to mix the serum tubes? (English)
Abstract [sv]

Bakgrund: Cirka 2/3 av alla preanalytiska fel uppkommer i den preanalytiska fasen, där blandning av provrör direkt efter provtagningen är en av faktorerna. Studiens syfte var att jämföra analysresultaten för glukos, laktatdehydrogenas samt kalium i serum från blandade rör med oblandade rör. Studiens syfte var även att undersöka hållbarheten på dessa tre analyter i rumstemperatur (25 °C) under sju dagar.

Metod: 100 blandade och 100 oblandade serumrör med gel, från 100 patienter, analyserades på Architect ci2800. Analysresultaten jämfördes statistiskt med parat t-test. Alla serumrör förvarades i rumstemperatur under sju dagar och de tre analyterna analyserades dagligen. Analysresultaten jämfördes statistiskt med ANOVA.

Resultat: Studien visade ingen statistiskt signifikant skillnad (p > 0,05) för glukos samt kalium mellan de oblandade och blandade rören. Laktatdehydrogenas uppvisar en statistiskt signifikant skillnad (p < 0,05) mellan de oblandade och blandade rören. Skillnaderna i hållbarheten mellan dag 1, dag 2 och dag 7 för glukos, laktatdehydrogenas och kalium visade sig vara statistiskt signifikanta (p < 0,05).

Slutsats: Studien indikerar att det inte är nödvändigt att blanda serumrören direkt efter provtagningen men det är viktigt att blanda andra typer av rör, exempelvis citratrör, därför är det bra att skapa sig en rutin då man blandar alla rör. Glukos är den mest stabila analyten i rumstemperatur i sju dagar medan kalium är den minst stabila.

Abstract [en]

Background: Approximately 2/3 of all pre-analytical errors occur in the pre-analytical phase, where mixing of sample tubes directly after sampling is one of the factors. The purpose of the study was to compare the analytical results for the glucose, lactate dehydrogenase and potassium in serum from mixed tubes with unmixed tubes. The study's aim was also to investigate the durability of these three analytes at room temperature (25 °C) for seven days.

Method: 100 mixed and 100 unmixed serum tubes with gel, from 100 patients, were analyzed on Architect ci2800. Assay results were statistically compared with the paired t-test. All the serum tubes were stored at room temperature for seven days and the three analytes were analyzed daily. The results of the analysis were compared statistically by ANOVA.

Results: No statistical significant difference (p > 0,05) was found for glucose and potassium between the unmixed and mixed tubes. Lactate dehydrogenase showed a small but statistical significant difference (p < 0,05) between the unmixed and mixed tubes. The differences in durability between day 1, day 2 and day 7 of the glucose, lactate dehydrogenase and potassium was found to be statistic significant (p < 0,05).

Conclusion: This study indicates that it is not necessary to mix the serum tubes immediately after the blood sampling, however it is important to mix other types of tubes, such as citrate tube. Therefore it is good to create a routine while you are mixing all the tubes. Glucose is the most stable analyte in room temperature for seven days while the potassium is the least stable.

Place, publisher, year, edition, pages
2014. , 38 p.
Keyword [en]
pre-analytical errors, mixing, serum tubes, durability
Keyword [sv]
preanalytiska fel, blandning, serumrör, hållbarhet
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:liu:diva-111914ISRN: LIU-HU/BML-G–14/020–SEOAI: oai:DiVA.org:liu-111914DiVA: diva2:761875
External cooperation
LaboratorieMedicinskt Centrum Gotland
Subject / course
Biomedical Laboratory Science
Supervisors
Examiners
Available from: 2015-01-14 Created: 2014-11-09 Last updated: 2015-01-14Bibliographically approved

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