Regulatory T cells in children with type 1 diabetes
(English)Manuscript (Other academic)
Introduction Function and frequency of CD4+CD25high T cells in type 1 diabetes (T1D) patients has been studied in cross-sectional studies. We wanted to investigate the changes in number and function of CD4+CD25high T cells in children with T1D during follow-up.
Material and Method The study includes in total 35 children with T1D and 17 healthy children. The number of CD4+CD25high regulatory T cells and their CTLA-4 expression was analyzed by flow cytometry and the function as the effect of CD4+CD25high T cells on proliferative and cytokine response after mitogen stimulation.
Results We found no differences between healthy and diabetic children in the amount of CD4+CD25highCTLA-4+ regulatory T cells and the numbers did not change during 18 months follow-up. We observed higher median fluorescence intensity of intracellular CTLA-4 on CD4+CD25high T cells in the diabetic children at diagnos compared to healthy children. Proliferation response to PHA was higher in the CD25depleted PBMC than in the whole PBMC population in diabetic and healthy children. Children with T1D showed a higher spontaneous secretion of IL-10 and TGF-β and higher expression of IFN-γ specific mRNA among CD25depleted PBMC than was seen in healthy children.
Conclusion Our data indicate that children with T1D have similar amounts of circulating CD4+CD25high regulatory cells as healthy children. Our data further suggest an increased activity among regulatory T cells in children with T1D.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-12439OAI: oai:DiVA.org:liu-12439DiVA: diva2:77