liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Early cytokine release in response to live Borrelia burgdorferi Sensu Lato Spirochetes is largely complement independent
Linneaues University, Kalmar .
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Ryhov County Hospital Jönköping .
Linneaues University, Kalmar.
University of Umeå .
Show others and affiliations
2014 (English)In: PloS one, ISSN 1932-6203, Vol. 9, no 9, e108013- p.Article in journal (Refereed) Published
Abstract [en]

AIM: Here we investigated the role of complement activation in phagocytosis and the release of cytokines and chemokines in response to two clinical isolates: Borrelia afzelii K78, which is resistant to complement-mediated lysis, and Borrelia garinii LU59, which is complement-sensitive.

METHODS: Borrelia spirochetes were incubated in hirudin plasma, or hirudin-anticoagulated whole blood. Complement activation was measured as the generation of C3a and sC5b-9. Binding of the complement components C3, factor H, C4, and C4BP to the bacterial surfaces was analyzed. The importance of complement activation on phagocytosis, and on the release of cytokines and chemokines, was investigated using inhibitors acting at different levels of the complement cascade.

RESULTS: 1) Borrelia garinii LU59 induced significantly higher complement activation than did Borrelia afzelii K78. 2) Borrelia afzelii K78 recruited higher amounts of factor H resulting in significantly lower C3 binding. 3) Both Borrelia strains were efficiently phagocytized by granulocytes and monocytes, with substantial inhibition by complement blockade at the levels of C3 and C5. 4) The release of the pro-inflammatory cytokines and chemokines IL-1β, IL-6, TNF, CCL20, and CXCL8, together with the anti-inflammatory IL-10, were increased the most (by>10-fold after exposure to Borrelia). 5) Both strains induced a similar release of cytokines and chemokines, which in contrast to the phagocytosis, was almost totally unaffected by complement blockade.

CONCLUSIONS: Our results show that complement activation plays an important role in the process of phagocytosis but not in the subsequent cytokine release in response to live Borrelia spirochetes.

Place, publisher, year, edition, pages
Public Library of Science , 2014. Vol. 9, no 9, e108013- p.
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:liu:diva-112749DOI: 10.1371/journal.pone.0108013ISI: 000345745400038PubMedID: 25265036OAI: oai:DiVA.org:liu-112749DiVA: diva2:771018
Available from: 2014-12-12 Created: 2014-12-12 Last updated: 2015-10-09

Open Access in DiVA

fulltext(612 kB)62 downloads
File information
File name FULLTEXT01.pdfFile size 612 kBChecksum SHA-512
53471cc1f10a6b82df352cd17d2a83e1cdfd618dc742f7f3772b82daa5125744e7e1acc11f4e664cba41a78a541a0857f4e025a19cc61f258d7827b79b2f1246
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Henningsson, Anna JForsberg, PiaErnerudh, Jan

Search in DiVA

By author/editor
Henningsson, Anna JForsberg, PiaErnerudh, Jan
By organisation
Division of Microbiology and Molecular MedicineFaculty of Health SciencesDepartment of Infectious DiseasesDivision of Inflammation MedicineDepartment of Clinical Immunology and Transfusion Medicine
Infectious Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 62 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 140 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf