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Evaluation of High-Resolution Melting Curve Analysis of Ligation-Mediated Real-Time PCR, a Rapid Method for Epidemiological Typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) Pathogens
Kalmar County Hospital, Sweden; Linnaeus University, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
Public Health Agency Sweden, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Heart and Medicine Center, Department of Infectious Diseases.
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2014 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 52, no 12, 4339-4342 p.Article in journal (Refereed) Published
Abstract [en]

A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.

Place, publisher, year, edition, pages
American Society for Microbiology , 2014. Vol. 52, no 12, 4339-4342 p.
National Category
Clinical Medicine Basic Medicine
Identifiers
URN: urn:nbn:se:liu:diva-113004DOI: 10.1128/JCM.02537-14ISI: 000345222900033PubMedID: 25232168OAI: oai:DiVA.org:liu-113004DiVA: diva2:779085
Note

Funding Agencies|Marianne and Marcus Wallenberg Foundation; FORSS (The Research Council of Southeast Sweden)

Available from: 2015-01-12 Created: 2015-01-08 Last updated: 2017-12-05

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Ryberg, AnnaHällgren, AnitaNilsson, Lennart ESchön, Thomas

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