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Visualization of oxidative stress in ex vivo biopsies using electron paramagnetic resonance imaging
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Biomedical Engineering. Linköping University, Center for Medical Image Science and Visualization (CMIV). (CMIV)
Linköping University, Department of Clinical and Experimental Medicine, Division of Neuro and Inflammation Science. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Pathology and Clinical Genetics.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology. Norwegian University of Science and Technology, Norway.
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Health Sciences. Linköping University, Center for Medical Image Science and Visualization (CMIV).
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2015 (English)In: Magnetic Resonance in Medicine, ISSN 0740-3194, E-ISSN 1522-2594, Vol. 73, no 4, 1682-1691 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE: The purpose of this study was to develop an X-Band electron paramagnetic resonance imaging protocol for visualization of oxidative stress in biopsies.

METHODS: The developed electron paramagnetic resonance imaging protocol was based on spin trapping with the cyclic hydroxylamine spin probe 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine and X-Band EPR imaging. Computer software was developed for deconvolution and back-projection of the EPR image. A phantom containing radicals of known spatial characteristic was used for evaluation of the developed protocol. As a demonstration of the technique electron paramagnetic resonance imaging of oxidative stress was performed in six sections of atherosclerotic plaques. Histopathological analyses were performed on adjoining sections.

RESULTS: The developed computer software for deconvolution and back-projection of the EPR images could accurately reproduce the shape of a phantom of known spatial distribution of radicals. The developed protocol could successfully be used to image oxidative stress in six sections of the three ex vivo atherosclerotic plaques.

CONCLUSIONS: We have shown that oxidative stress can be imaged using a combination of spin trapping with the cyclic hydroxylamine spin probe cyclic hydroxylamine spin probe 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine and X-Band EPR imaging. A thorough and systematic evaluation on different types of biopsies must be performed in the future to validate the proposed technique. Magn Reson Med, 2014.

Place, publisher, year, edition, pages
2015. Vol. 73, no 4, 1682-1691 p.
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Clinical Medicine
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URN: urn:nbn:se:liu:diva-113407DOI: 10.1002/mrm.25267ISI: 000351685900035PubMedID: 24753234OAI: oai:DiVA.org:liu-113407DiVA: diva2:781763
Available from: 2015-01-19 Created: 2015-01-19 Last updated: 2017-12-05

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Gustafsson, HåkanHallbeck, MartinLindgren, MikaelKolbun, NatalliaEngström, Mariade Muinck, EboZachrisson, Helene

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Gustafsson, HåkanHallbeck, MartinLindgren, MikaelKolbun, NatalliaEngström, Mariade Muinck, EboZachrisson, Helene
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Division of Radiological SciencesFaculty of Health SciencesDepartment of Biomedical EngineeringCenter for Medical Image Science and Visualization (CMIV)Division of Neuro and Inflammation ScienceFaculty of Medicine and Health SciencesDepartment of Clinical Pathology and Clinical GeneticsChemistryThe Institute of TechnologyDivision of Cardiovascular MedicineDepartment of Cardiology in LinköpingDepartment of Clinical Physiology in Linköping
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