Monitoring of autophagy is complicated: Salinomycin as an example
2015 (English)In: Biochimica et Biophysica Acta. Molecular Cell Research, ISSN 0167-4889, E-ISSN 1879-2596, ISSN 0167-4889, Vol. 1853, no 3, 604-610 p.Article in journal (Refereed) Published
Monitoring of autophagy is challenging because of its multiple steps and lack of single befitting technique for a complete mechanistic understanding, which makes the task complicated. Here, we evaluate the functionality of autophagy triggered by salinomycin (anti-cancer stem cell agent) using flow cytometry and advanced microscopy. We show that salinomycin does induce functional autophagy at lower concentrations and such a dose is cell type-dependent. For example, PC3 cells show active autophagic flux at 10μM concentration of salinomycin while murine embryonic fibroblasts already show an inhibition of flux at such doses. A higher concentration of salinomycin (i.e. 30μM) inhibits autophagic flux in both cell types. The data confirms our previous findings that salinomycin is an inducer of autophagy, whereas autophagic flux inhibition is a secondary response.
Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 1853, no 3, 604-610 p.
Autophagic flux; GFP-LC3; Salinomycin; Vacuolization; mTandem GFP-RFP LC3; p62/SQSTRM1
Basic Medicine Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:liu:diva-113565DOI: 10.1016/j.bbamcr.2014.12.022ISI: 000349878500007PubMedID: 25541282OAI: oai:DiVA.org:liu-113565DiVA: diva2:783020