The growth factor-like adipokine tartrate-resistant acid phosphatase 5a interacts with the rod G3 domain of adipocyte-produced nidogen-2
2014 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 454, no 3, 446-452 p.Article in journal (Refereed) Published
The adipoldne tartrate resistant acid phosphatase (TRAP) 5a isoform exerts a growth factor-effect on pre-adipocytes. This study aimed to identify potential TRAP 5a interacting proteins in pre-adipocytes using pull down assays in combination with mass spectrometry. Nidogen-2, a protein shown to be expressed intracellularly and for secretion by pre-adipocytes, was shown to interact, through its globular G3 domain, with TRAP 5a in vitro. In vivo, TRAP 5a interacted with nidogen-2 in cultured 3T3-L1 mouse pre-adipocytes, as well as with transforming growth factor-beta (TGF-beta) interacting protein (TRIP-1), which is a protein that has previously been suggested to interact with TRAP in bone. In addition, TRAP 5a and nidogen-2 co-localized in adipose tissue cells in situ. These results indicate that TRAP 5a interacts with nidogen-2 and TRIP-1 in pre-adipocytic cells.
Place, publisher, year, edition, pages
Elsevier , 2014. Vol. 454, no 3, 446-452 p.
Tartrate resistant acid phosphatase; Acid phosphatase type 5; Nidogen-2; Extracellular matrix; Pre-adipocytes; Growth factor
IdentifiersURN: urn:nbn:se:liu:diva-113581DOI: 10.1016/j.bbrc.2014.10.112ISI: 000346690600016PubMedID: 25450682OAI: oai:DiVA.org:liu-113581DiVA: diva2:783026
Funding Agencies|Swedish Research Council; KI fonder2015-01-232015-01-232015-01-23