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The growth factor-like adipokine tartrate-resistant acid phosphatase 5a interacts with the rod G3 domain of adipocyte-produced nidogen-2
Karolinska University, Sweden.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Karolinska University, Sweden.
Karolinska University, Sweden.
2014 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 454, no 3, 446-452 p.Article in journal (Refereed) Published
Abstract [en]

The adipoldne tartrate resistant acid phosphatase (TRAP) 5a isoform exerts a growth factor-effect on pre-adipocytes. This study aimed to identify potential TRAP 5a interacting proteins in pre-adipocytes using pull down assays in combination with mass spectrometry. Nidogen-2, a protein shown to be expressed intracellularly and for secretion by pre-adipocytes, was shown to interact, through its globular G3 domain, with TRAP 5a in vitro. In vivo, TRAP 5a interacted with nidogen-2 in cultured 3T3-L1 mouse pre-adipocytes, as well as with transforming growth factor-beta (TGF-beta) interacting protein (TRIP-1), which is a protein that has previously been suggested to interact with TRAP in bone. In addition, TRAP 5a and nidogen-2 co-localized in adipose tissue cells in situ. These results indicate that TRAP 5a interacts with nidogen-2 and TRIP-1 in pre-adipocytic cells.

Place, publisher, year, edition, pages
Elsevier , 2014. Vol. 454, no 3, 446-452 p.
Keyword [en]
Tartrate resistant acid phosphatase; Acid phosphatase type 5; Nidogen-2; Extracellular matrix; Pre-adipocytes; Growth factor
National Category
Chemical Sciences
URN: urn:nbn:se:liu:diva-113581DOI: 10.1016/j.bbrc.2014.10.112ISI: 000346690600016PubMedID: 25450682OAI: diva2:783026

Funding Agencies|Swedish Research Council; KI fonder

Available from: 2015-01-23 Created: 2015-01-23 Last updated: 2015-01-23

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