liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
L-Cystathionine Inhibits the Mitochondria-Mediated Macrophage Apoptosis Induced by Oxidized Low Density Lipoprotein
Peking University, Peoples R China.
Peking University, Peoples R China; Minist Educ, Peoples R China.
Peking University, Peoples R China.
Show others and affiliations
2014 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 15, no 12, 23059-23073 p.Article in journal (Refereed) Published
Abstract [en]

This study was designed to investigate the regulatory role of L-cystathionine in human macrophage apoptosis induced by oxidized low density lipoprotein (ox-LDL) and its possible mechanisms. THP-1 cells were induced with phorbol 12-myristate 13-acetate (PMA) and differentiated into macrophages. Macrophages were incubated with ox-LDL after pretreatment with L-cystathionine. Superoxide anion, apoptosis, mitochondrial membrane potential, and mitochondrial permeability transition pore (MPTP) opening were examined. Caspase-9 activities and expression of cleaved caspase-3 were measured. The results showed that compared with control group, ox-LDL treatment significantly promoted superoxide anion generation, release of cytochrome c (cytc) from mitochondrion into cytoplasm, caspase-9 activities, cleavage of caspase-3, and cell apoptosis, in addition to reduced mitochondrial membrane potential as well as increased MPTP opening. However, 0.3 and 1.0 mmol/L L-cystathionine significantly reduced superoxide anion generation, increased mitochondrial membrane potential, and markedly decreased MPTP opening in ox-LDL + L-cystathionine macrophages. Moreover, compared to ox-LDL treated-cells, release of cytc from mitochondrion into cytoplasm, caspase-9 activities, cleavage of caspase-3, and apoptosis levels in L-cystathionine pretreated cells were profoundly attenuated. Taken together, our results suggested that L-cystathionine could antagonize mitochondria-mediated human macrophage apoptosis induced by ox-LDL via inhibition of cytc release and caspase activation.

Place, publisher, year, edition, pages
MDPI , 2014. Vol. 15, no 12, 23059-23073 p.
Keyword [en]
L-cystathionine; ox-LDL; macrophage; mitochondrial; oxidative stress; apoptosis; caspase-9; caspase-3
National Category
Basic Medicine
Identifiers
URN: urn:nbn:se:liu:diva-113576DOI: 10.3390/ijms151223059ISI: 000346797400089PubMedID: 25514411OAI: oai:DiVA.org:liu-113576DiVA: diva2:783083
Note

Funding Agencies|Major Basic Research Development Program of Peoples Republic of China [2012CB517806, 2013CB933801, 2011CB503904]; National Natural Science Foundation of China [31130030, 81370154, 81121061]; Grant of Ministry of Education, China [20130001120047]; Beijing Natural Science Foundation [7121014]; Key Laboratory of Remodeling-related Cardiovascular Diseases, Ministry of Education [2014XXGB02]

Available from: 2015-01-23 Created: 2015-01-23 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(2770 kB)122 downloads
File information
File name FULLTEXT01.pdfFile size 2770 kBChecksum SHA-512
4258e080f3df1deb868362a8ad3f4df1fac5100d602fe6cc73c411cc18761c5464a96d9a7da916992be34c96ec20cada73e3ae069004c54db8e2e52efc64137b
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Holmberg, Lukas

Search in DiVA

By author/editor
Holmberg, Lukas
By organisation
Division of Cell BiologyFaculty of Medicine and Health Sciences
In the same journal
International Journal of Molecular Sciences
Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 122 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 86 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf