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Thyroid hormone does not induce maturation of embryonicchicken cardiomyocytes in vitro
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, The Institute of Technology.
2014 (English)In: Physiological Reports, E-ISSN 2051-817X, Vol. 2, no 12, e12182- p.Article in journal (Refereed) Published
Abstract [sv]

Fetal cardiac growth in mammalian models occurs primarily by cell proliferation(hyperplasia). However, most cardiomyocytes lose the ability to proliferateclose to term and heart growth continues by increasing cell size(hypertrophy). In mammals, the thyroid hormone triiodothyronine (T3) is animportant driver of this process. Chicken cardiomyocytes, however, keep theirproliferating ability long after hatching but little information is available onthe mechanisms controlling cell growth and myocyte maturation in thechicken heart. Our aim was to study the role of T3 on proliferation and differentiationof embryonic chicken cardiomyocytes (ECCM), enzymaticallyisolated from 19-day-old embryos and to compare the effects to those of insulin-like growth factor-1 (IGF-1) and phenylephrine (PE). Hyperplasia wasmeasured using a proliferation assay (MTS) and hypertrophy/multinucleationwas analyzed morphologically by phalloidin staining of F-actin and nuclearstaining with DAPI. We show that IGF-1 induces a significant increase inECCM proliferation (30%) which is absent with T3 and PE. PE induced bothhypertrophy (61%) and multinucleation (41%) but IGF-1 or T3 did not. Inconclusion, we show that T3 does not induce maturation or proliferation ofcardiomyocytes, while IGF-1 induces cardiomyocyte proliferation and PEinduces maturation of cardiomyocytes.

Place, publisher, year, edition, pages
Wiley Periodicals, Inc. , 2014. Vol. 2, no 12, e12182- p.
Keyword [en]
Heart growth, hyperplasia, hypertrophy, insulin-like growth factor-1, thyroid hormone
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:liu:diva-113604DOI: 10.14814/phy2.12182PubMedID: 25501434OAI: oai:DiVA.org:liu-113604DiVA: diva2:783316
Available from: 2015-01-26 Created: 2015-01-26 Last updated: 2017-12-05Bibliographically approved

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Svensson Holm, Ann-Charlotte BLindgren, IsaÖsterman, HannaAltimiras, Jordi

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