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Cholesterol Oxidase Functionalised Polyaniline/Carbon Nanotube Hybrids for an Amperometric Biosensor
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-1815-9699
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
2015 (English)In: Journal of Nanoscience and Nanotechnology, ISSN 1533-4880, E-ISSN 1533-4899, Vol. 15, no 5, 3373-3377 p.Article in journal (Refereed) Published
Abstract [en]

Functional carbon nanotubes (CNT) have attracted much attention for analytical and biomedical applications. This paper describes the fabrication of a cholesterol oxidase (ChOx) immobilised polyaniline (PANI)/CNT composite electrode for the amperometric detection of cholesterol. The prepared ChOx/PANI/CNT/Au bioelectrode bound ChOx via the available functionalties of PANI (-NH2) and CNT (-COOH). Moreover, the CNT creates a network inside the matrix that strengthens the mechanical property of the bioelectrode. The multifunctional matrix is presumed to provide a 3D-mesoporous surface, which substantially enhances enzyme activity. The linear range of the biosensor for cholesterol oleate was 30-280 mu M with a response time of 10 sec.

Place, publisher, year, edition, pages
American Scientific Publishers , 2015. Vol. 15, no 5, 3373-3377 p.
Keyword [en]
Polyaniline; Carbon Nanotube; Enzyme Immobilisation; Cholesterol Biosensor
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:liu:diva-114007DOI: 10.1166/jnn.2015.10209ISI: 000347435200008OAI: oai:DiVA.org:liu-114007DiVA: diva2:786831
Note

Funding Agencies|Swedish research Council [VR-2011-6058357]; European Commission [PIIF-GA-2009-254955]; IGEN; LIST; Linkoping University

Available from: 2015-02-06 Created: 2015-02-05 Last updated: 2017-12-04

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Turner, AnthonyTiwari, Ashutosh

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