liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
1H NMR determination of urinary betaine in patients with premature vascular disease and mild homocysteinemia
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Health Sciences. Linköping University, Center for Medical Image Science and Visualization (CMIV). Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Radiation Physics. Department of Biochemistry, University of Sydney, Australia.ORCID iD: 0000-0001-8661-2232
Department of Cardiovascular Medicine, Prince Henry Hospital, University of New South Wales, Australia.
Department of Biochemistry, University of Sydney, Australia.
Present address: Department of Physical Chemistry, University of Umeå, Umeå, Sweden.
1995 (English)In: Clinical Chemistry, ISSN 0009-9147, E-ISSN 1530-8561, Vol. 41, no 2, 275-283 p.Article in journal (Refereed) Published
Abstract [en]

Urinary N,N,N-trimethylglycine (betaine) and N,N-dimethylglycine (DMG) have been identified and quantified for clinical purposes by proton nuclear magnetic resonance (1H NMR) measurement in previous studies. We have assessed these procedures by using both one-dimensional (1-D) and 2-D NMR spectroscopy, together with pH titration of urinary extracts to help assign 1H NMR spectral peaks. The betaine calibration curve linearity was excellent (r = 0.997, P = 0.0001) over the concentration range 0.2-1.2 mmol/L, and CVs for replicate betaine analyses ranged from 7% (n = 10) at the lowest concentration to 1% (n = 9) at the highest. The detection limit for betaine was < 15 mumol/L. Urinary DMG concentrations were substantially lower than those of betaine. Urinary betaine and DMG concentrations measured by 1H NMR spectroscopy from 13 patients with premature vascular disease and 17 normal controls provided clinically pertinent data. We conclude that 1H NMR provides unique advantages as a research tool for determination of urinary betaine and DMG concentrations.

Place, publisher, year, edition, pages
1995. Vol. 41, no 2, 275-283 p.
Keyword [en]
Adult, Amino Acid Metabolism, Inborn Errors/urine, Betaine/*urine, Creatinine/urine, Glycine/urine, Homocysteine/*blood, Humans, Hydrogen-Ion Concentration, *Magnetic Resonance Spectroscopy, Male, Regression Analysis, Sarcosine/analogs & derivatives/urine, Vascular Diseases/*urine
National Category
Physical Chemistry Chemical Sciences
URN: urn:nbn:se:liu:diva-114156PubMedID: 7533065OAI: diva2:790546

0009-9147 (Print) Journal Article Research Support, Non-U.S. Gov't

Available from: 2015-02-25 Created: 2015-02-11 Last updated: 2015-03-05Bibliographically approved

Open Access in DiVA

No full text

Other links

PubMedlänk till artikeln

Search in DiVA

By author/editor
Lundberg, Peter
By organisation
Division of Radiological SciencesFaculty of Health SciencesCenter for Medical Image Science and Visualization (CMIV)Department of Radiation Physics
In the same journal
Clinical Chemistry
Physical ChemistryChemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 44 hits
ReferencesLink to record
Permanent link

Direct link