Intracellular analysis of a lymphoblastoid cell line using H-NMR
1994 (English)In: Animal Cell Technology: Basic & Applied Aspects / [ed] T. Kobayashi, Y. Kitagawa & K. Okumura, Springer Verlag (Kluwer) , 1994, 351-358 p.Chapter in book (Refereed)
A complete analysis of growth and protein production characteristics of a commercially relevant animal cell line has been undertaken. The model cell line was a human lymphoblastoid which produces an IgG antibody against anti-D in blood. The batch growth characteristics of this cell line under different conditions was monitored and analysis of major nutrients and products as well as amino acids in the medium was undertaken. The internal (i.e. intracellular) concentrations of a variety of species in the cell were investigated using H-NMR of cellular extracts. This analysis enabled the quantitative determination of many amino acids, biosynthetic intermediates, secreted products and energetically important intermediates.
Place, publisher, year, edition, pages
Springer Verlag (Kluwer) , 1994. 351-358 p.
Physical Chemistry Chemical Sciences
IdentifiersURN: urn:nbn:se:liu:diva-114158DOI: 10.1007/978-94-011-0848-5_53ISBN: 978-94-010-4366-3 (print)ISBN: 978-94-011-0848-5 (online)OAI: oai:DiVA.org:liu-114158DiVA: diva2:790547