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Intracellular analysis of a lymphoblastoid cell line using H-NMR
Departments of Chemical Engineering, University of Sydney, Australia.
Departments of Chemical Engineering, University of Sydney, Australia.
Departments of Infectious Diseases, University of Sydney, Australia.
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Health Sciences. Linköping University, Center for Medical Image Science and Visualization (CMIV). Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Radiation Physics. Departments of Biochemistry, University of Sydney, Australia .ORCID iD: 0000-0001-8661-2232
1994 (English)In: Animal Cell Technology: Basic & Applied Aspects / [ed] T. Kobayashi, Y. Kitagawa & K. Okumura, Springer Verlag (Kluwer) , 1994, 351-358 p.Chapter in book (Refereed)
Abstract [en]

A complete analysis of growth and protein production characteristics of a commercially relevant animal cell line has been undertaken. The model cell line was a human lymphoblastoid which produces an IgG antibody against anti-D in blood. The batch growth characteristics of this cell line under different conditions was monitored and analysis of major nutrients and products as well as amino acids in the medium was undertaken. The internal (i.e. intracellular) concentrations of a variety of species in the cell were investigated using H-NMR of cellular extracts. This analysis enabled the quantitative determination of many amino acids, biosynthetic intermediates, secreted products and energetically important intermediates.

Place, publisher, year, edition, pages
Springer Verlag (Kluwer) , 1994. 351-358 p.
National Category
Physical Chemistry Chemical Sciences
URN: urn:nbn:se:liu:diva-114158DOI: 10.1007/978-94-011-0848-5_53ISBN: 978-94-010-4366-3 (print)ISBN: 978-94-011-0848-5 (online)OAI: diva2:790547
Available from: 2015-02-25 Created: 2015-02-11 Last updated: 2015-03-05Bibliographically approved

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Lundberg, Peter
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Division of Radiological SciencesFaculty of Health SciencesCenter for Medical Image Science and Visualization (CMIV)Department of Radiation Physics
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ReferencesLink to record
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