liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Diffusion of solutes in agarose and alginate gels: 1H and 23Na PFGSE and 23Na TQF NMR studies
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Health Sciences. Linköping University, Center for Medical Image Science and Visualization (CMIV). Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Radiation Physics. Department of Physical Chemistry, University of Umeå, Umeå, Sweden / Department of Biochemistry, University of Sydney, Australia .ORCID iD: 0000-0001-8661-223
Department of Physical Chemistry, University of Umeå, Umeå, Sweden / Department of Biochemistry, University of Sydney, Australia.
1997 (English)In: Magnetic Resonance in Medicine, ISSN 0740-3194, E-ISSN 1522-2594, Vol. 37, no 1, 44-52 p.Article in journal (Refereed) Published
Abstract [en]

Cells immobilized in gels experience potential metabolic restrictions in the form of reduced diffusion rates of metabolites and ions and their possible selective adsorption on the gel matrix. Diffusion and relaxation characteristics of common solutes in agarose and barium alginate gels were investigated at 37 degrees C by using 1H PFGSE and 23Na TQF NMR spectroscopy. Glucose, glycine, alanine, lactate, sodium ions, and HDO were studied. There were no selective interactions between any of the metabolites and the gel materials but the diffusion coefficients were uniformly reduced. The effects of metabolite diffusion and utilization, in gel beads and threads containing cells, were simulated by using a reaction diffusion model incorporating the measured diffusion coefficients. Metabolism is expected to be very significantly limited by diffusion of solutes to and from the cells that are centrally located within gel threads or spheres of radius approximately 2.0 mm, which is a commonly used size.

Place, publisher, year, edition, pages
John Wiley & Sons, 1997. Vol. 37, no 1, 44-52 p.
Keyword [en]
Alginates chemistry, Animals, Cattle, Cells, Immobilized, Diffusion, Gels, Glucuronic Acid, Hexuronic Acids, Magnetic Resonance Spectroscopy/*methods, Sepharose/*chemistry, Serum Albumin, Bovine
National Category
Physical Chemistry Chemical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-114154DOI: 10.1002/mrm.1910370108PubMedID: 8978631OAI: oai:DiVA.org:liu-114154DiVA: diva2:790552
Note

0740-3194 (Print) Journal Article

Available from: 2015-02-25 Created: 2015-02-11 Last updated: 2017-12-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Lundberg, Peter

Search in DiVA

By author/editor
Lundberg, Peter
By organisation
Division of Radiological SciencesFaculty of Health SciencesCenter for Medical Image Science and Visualization (CMIV)Department of Radiation Physics
In the same journal
Magnetic Resonance in Medicine
Physical ChemistryChemical Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 54 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf