A phosphorus-31 nuclear magnetic resonance study of elicitor-mediated metabolic changes in Catharanthus roseus suspension cultures
1997 (English)In: In vitro cellular & developmental biology. Plant, ISSN 1054-5476, E-ISSN 1475-2689, Vol. 33, no 4, 301-305 p.Article in journal (Refereed) Published
The induction of metabolic changes in suspension cultured cells of Catharanthus roseus upon elicitation has been investigated. Addition of a yeast glucan preparation to the growth medium resulted in induction of phenylalanine ammonia lyase. Phosphate uptake and metabolism of elicited cells was followed by 31P nuclear magnetic resonance. The uptake rate of Pi from the medium by oxygenated cells of C. roseus was reduced immediately after elicitation. Despite this reduced Pi uptake elicited cells had significantly increased amounts of ATP (twofold increase within 6 h). Cytoplasmic levels of Pi, phosphomonoesters, and Uridine Diphasphate glucose (UDP-Glc) were unaffected by eliciation. Furthermore, the cytoplasmic and vacuolar pH remained constant after addition of elicitor.
Place, publisher, year, edition, pages
Springer, 1997. Vol. 33, no 4, 301-305 p.
Physical Chemistry Chemical Sciences
IdentifiersURN: urn:nbn:se:liu:diva-114153DOI: 10.1007/s11627-997-0055-1OAI: oai:DiVA.org:liu-114153DiVA: diva2:790554
Times Cited: 2 Lundberg, P Vogel, HJ Brodelius, PE 22015-02-252015-02-112015-03-05Bibliographically approved