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NMR studies of erythrocytes immobilized in agarose and alginate gels
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Health Sciences. Linköping University, Center for Medical Image Science and Visualization (CMIV). Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Radiation Physics. Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia.ORCID iD: 0000-0001-8661-2232
Division of Science and Technology, Griffith University, Nathan, Queensland, 4111, Australia.
Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia.
Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia.
1992 (English)In: Magnetic Resonance in Medicine, ISSN 0740-3194, E-ISSN 1522-2594, Magn Reson Med, Vol. 25, no 2, 273-288 p.Article in journal (Refereed) Published
Abstract [en]

31P and 13C NMR were used to study the energy metabolism in perfused, human erythrocytes. The erythrocytes were immobilized in agarose threads, Ca- or Ba-alginate beads, and Ba-alginate-coated agarose threads. Erythrocytes were easily washed out from the agarose threads, but not from alginate-containing gels. Various small molecules, such as hypophosphite, dimethyl methylphosphonate, and methylphosphonate, were taken up from the perfusion medium in a normal manner. In addition, the 2,3-bisphosphoglycerate (2,3-DPG) chemical shifts were sensitive to the oxygen partial pressure suggesting that O2 molecules were diffusing through the gel and modifying the binding of 2,3-DPG to hemoglobin. A combination of inosine and pyruvate stimulated the synthesis of 2,3-DPG, but only if inorganic phosphate was present in the perfusion medium. Inosine only resulted in a dramatic rise in the intracellular sugarphosphate concentrations. Furthermore, [2-13C]glucose was converted to [2-13C]lactate by immobilized cells at a rate which was comparable to that in a control suspension. In summary, immobilization in Ba-alginate-coated agarose threads was an efficient way of trapping human erythrocytes for whole cell NMR investigations.

Place, publisher, year, edition, pages
John Wiley & Sons, 1992. Vol. 25, no 2, 273-288 p.
Keyword [en]
Alginates, Diphosphoglyceric Acids/*metabolism, Energy Metabolism/drug effects/*physiology, Erythrocytes/*metabolism, Gels, Humans, Inosine/pharmacology, Magnetic Resonance Spectroscopy/*methods, Oxygen/physiology, Partial Pressure, Pyruvates/pharmacology, Sepharose
National Category
Physical Chemistry
URN: urn:nbn:se:liu:diva-114163DOI: 10.1002/mrm.1910250206PubMedID: 1614311OAI: diva2:790757

0740-3194 (Print) Comparative Study In Vitro Journal Article Research Support, Non-U.S. Gov't

Available from: 2015-02-25 Created: 2015-02-11 Last updated: 2015-03-05Bibliographically approved

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Lundberg, Peter
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Division of Radiological SciencesFaculty of Health SciencesCenter for Medical Image Science and Visualization (CMIV)Department of Radiation Physics
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Magnetic Resonance in Medicine
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