NMR studies of erythrocytes immobilized in agarose and alginate gels
1992 (English)In: Magnetic Resonance in Medicine, ISSN 0740-3194, E-ISSN 1522-2594, Magn Reson Med, Vol. 25, no 2, 273-288 p.Article in journal (Refereed) Published
31P and 13C NMR were used to study the energy metabolism in perfused, human erythrocytes. The erythrocytes were immobilized in agarose threads, Ca- or Ba-alginate beads, and Ba-alginate-coated agarose threads. Erythrocytes were easily washed out from the agarose threads, but not from alginate-containing gels. Various small molecules, such as hypophosphite, dimethyl methylphosphonate, and methylphosphonate, were taken up from the perfusion medium in a normal manner. In addition, the 2,3-bisphosphoglycerate (2,3-DPG) chemical shifts were sensitive to the oxygen partial pressure suggesting that O2 molecules were diffusing through the gel and modifying the binding of 2,3-DPG to hemoglobin. A combination of inosine and pyruvate stimulated the synthesis of 2,3-DPG, but only if inorganic phosphate was present in the perfusion medium. Inosine only resulted in a dramatic rise in the intracellular sugarphosphate concentrations. Furthermore, [2-13C]glucose was converted to [2-13C]lactate by immobilized cells at a rate which was comparable to that in a control suspension. In summary, immobilization in Ba-alginate-coated agarose threads was an efficient way of trapping human erythrocytes for whole cell NMR investigations.
Place, publisher, year, edition, pages
John Wiley & Sons, 1992. Vol. 25, no 2, 273-288 p.
Alginates, Diphosphoglyceric Acids/*metabolism, Energy Metabolism/drug effects/*physiology, Erythrocytes/*metabolism, Gels, Humans, Inosine/pharmacology, Magnetic Resonance Spectroscopy/*methods, Oxygen/physiology, Partial Pressure, Pyruvates/pharmacology, Sepharose
IdentifiersURN: urn:nbn:se:liu:diva-114163DOI: 10.1002/mrm.1910250206PubMedID: 1614311OAI: oai:DiVA.org:liu-114163DiVA: diva2:790757
0740-3194 (Print) Comparative Study In Vitro Journal Article Research Support, Non-U.S. Gov't2015-02-252015-02-112015-03-05Bibliographically approved