liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Post-mortem energy metabolism in bovine muscles studied by non-invasive phosphorus-31 nuclear magnetic resonance
Department of Physical Chemistry 2, University of Lund, Lund, Sweden.
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Health Sciences. Linköping University, Center for Medical Image Science and Visualization (CMIV). Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Radiation Physics. Department of Physical Chemistry 2, University of Lund, Lund, Sweden.ORCID iD: 0000-0001-8661-2232
Department of Physical Chemistry 2, University of Lund, Lund, Sweden.
Department of Physical Chemistry 2, University of Lund, Lund, Sweden.
Show others and affiliations
1985 (English)In: Meat Science, ISSN 0309-1740, Vol. 13, no 1, 1-18 p.Article in journal (Refereed) Published
Abstract [en]

Phosphorus-31 Nuclear Magnetic Resonance ((31)P-NMR) has been utilized to follow non-invasively the post-mortem metabolism of the major phosphorylated metabolites in muscles from beef slaughter carcasses. In addition to adenosine-5'-triphosphate (ATP), creatine phosphate (CP) and inorganic phosphate (P(i)) considerable amounts of glucose- and fructose-6-phosphate (G6P and F6P, respectively) as well as glycerol-3-phosphate (Glyc3P) were detected. ATP was mainly present as a Mg(2+)-ATP complex. Adenosine-5'-diphosphate (ADP) appeared to be mainly bound to muscle proteins. A good quantitative agreement was found for the levels of ATP, CP and sugar phosphates (SP) when estimated by NMR or enzymatic assays. Since the chemical shifts of the P(i) and sugar phosphate resonances are a function of the pH, the intracellular pH could be directly deduced from the NMR spectra. Values obtained in this manner were, within the errors of both methods, the same as those determined in iodoacetate/KCl homogenates. The pH gradients within the tissue never exceeded 0.3 pH units. In a final set of experiments we used (31)P-NMR 10 study the effects of electrical stimulation on the intracellular pH and post-mortem metabolism. It was concluded that (31)P-NMR, due to its non-invasive nature plus the fact that some of the NMR parameters are sensitive to the intracellular environment, provides a useful complement to existing methods for the study of post-mortem metabolism.

Place, publisher, year, edition, pages
1985. Vol. 13, no 1, 1-18 p.
National Category
Physical Chemistry
URN: urn:nbn:se:liu:diva-114178DOI: 10.1016/S0309-1740(85)80001-2OAI: diva2:790799

Times Cited: 22 Vogel, hj lundberg, p fabiansson, s ruderus, h tornberg, e 22

Available from: 2015-02-25 Created: 2015-02-11 Last updated: 2015-03-10Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Lundberg, Peter
By organisation
Division of Radiological SciencesFaculty of Health SciencesCenter for Medical Image Science and Visualization (CMIV)Department of Radiation Physics
In the same journal
Meat Science
Physical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 45 hits
ReferencesLink to record
Permanent link

Direct link