liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Measurement of Activity and Concentration of Paraoxonase 1 (PON-1) in Seminal Plasma and Identification of PON-2 in the Sperm of Boar Ejaculates
University of Murcia, Spain.
University of Murcia, Spain.
University of Murcia, Spain.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.
Show others and affiliations
2015 (English)In: Molecular Reproduction and Development, ISSN 1040-452X, E-ISSN 1098-2795, Vol. 82, no 1, 58-65 p.Article in journal (Refereed) Published
Abstract [en]

This study revealed and characterised the presence of the antioxidant enzymes paraoxonase (PON) type 1 (PON-1, extracellular) and type 2 (PON-2, intracellular) in boar semen. To evaluate PON-1, an entire ejaculate from each of ten boars was collected and the seminal plasma was harvested after double centrifugation (1,500g for 10min). Seminal plasma was analysed for concentration as well as enzymatic activity of PON-1 and total cholesterol levels. Seminal-plasma PON-1 concentration ranged from 0.961 to 1.670ng/ml while its enzymatic activity ranged from 0.056 to 0.400 IU/ml, which represent individual variance. Seminal-plasma PON-1 concentration and enzymatic activity were negatively correlated (r=-0.763; Pless than0.01). The activity of seminal-plasma PON-1 negatively correlated with ejaculate volume (r=-0.726, Pless than0.05), but positively correlated with sperm concentration (r=0.654, Pless than0.05). Total seminal-plasma cholesterol concentration positively correlated with PON-1 activity (r=0.773; Pless than0.01), but negatively correlated with PON-1 concentration (r=-0.709; Pless than0.05). The presence of intracellular PON-2 was determined via immunocytochemistry in spermatozoa derived from artificial insemination. PON-2 localised to the post-acrosomal area of the sperm head and principal piece of the tail in membrane-intact spermatozoa. In summary, PON is present in boar semen, with PON-1 at low levels in seminal plasma and PON-2 within the spermatozoa. Further studies are needed to characterise the relationship between antioxidant PONs with sperm and other seminal-plasma parameters. Mol. Reprod. Dev. 82: 58-65, 2015. (c) 2014 Wiley Periodicals, Inc.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2015. Vol. 82, no 1, 58-65 p.
National Category
Medical Bioscience
URN: urn:nbn:se:liu:diva-114595DOI: 10.1002/mrd.22444ISI: 000348656700007PubMedID: 25487823OAI: diva2:791430

Funding Agencies|Ministry of Economy and Competitiveness of Spain; European Regional Development Fund [AGL2012-39903]; Swedish Research Council Formas [2011-512]; Ministry of Education, Culture and Sport of Spain [FPU12/05008]

Available from: 2015-02-27 Created: 2015-02-26 Last updated: 2016-04-25

Open Access in DiVA

fulltext(349 kB)85 downloads
File information
File name FULLTEXT01.pdfFile size 349 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Rubér, MarieVicente Carrillo, AlejandroAtikuzzaman, MohammadRodriguez-Martinez, Heriberto
By organisation
Division of Clinical SciencesFaculty of Medicine and Health SciencesDivision of Microbiology and Molecular MedicineFaculty of Health Sciences
In the same journal
Molecular Reproduction and Development
Medical Bioscience

Search outside of DiVA

GoogleGoogle Scholar
Total: 85 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 210 hits
ReferencesLink to record
Permanent link

Direct link