liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Detection of a low-grade enteroviral infection in the islets of Langerhans of living patients newly diagnosed with type 1 diabetes.
Paediatric Dept., Oslo University Hospital, Norway.
Intervention Centre and Dept. of Surgery, Oslo University Hospital, and Faculty of Medicine, University of Oslo, Norway.
Dept. of Surgery, Oslo University Hospital, and Faculty of Medicine, University of Oslo, Norway.
Department of Immunology, Genetics and Pathology, Uppsala University, Sweden.
Show others and affiliations
2015 (English)In: Diabetes, ISSN 0012-1797, E-ISSN 1939-327X, Vol. 64, no 5, 1682-1687 p.Article in journal (Refereed) Published
Abstract [en]

The Diabetes Virus Detection study (DiViD) is the first to examine fresh pancreatic tissue at the diagnosis of type 1 diabetes for the presence of viruses. Minimal pancreatic tail resection was performed 3-9 weeks after onset of type 1 diabetes in 6 adult patients (age 24-35 years). The presence of enteroviral capsid protein 1 (VP1) and the expression of class I HLA were investigated by immunohistochemistry. Enterovirus RNA was analyzed from isolated pancreatic islets and from fresh frozen whole pancreatic tissue using PCR and sequencing. Non-diabetic organ donors served as controls. VP1 was detected in the islets of all type 1 diabetes patients (2 of 9 controls). Hyperexpression of class I HLA molecules was found in the islets of all patients (1 of 9 controls). Enterovirus specific RNA sequences were detected in 4 of 6 cases (0 of 6 controls). The results were confirmed in different laboratories. Only 1.7 % of the islets contained VP1 positive cells and the amount of enterovirus RNA was low. The results provides evidence for the presence of enterovirus in pancreatic islets of type 1 diabetic patients, being consistent with the possibility that a low grade enteroviral infection in the pancreatic islets contribute to disease progression in humans.

Place, publisher, year, edition, pages
American Diabetes Association , 2015. Vol. 64, no 5, 1682-1687 p.
National Category
Infectious Medicine
URN: urn:nbn:se:liu:diva-116469DOI: 10.2337/db14-1370ISI: 000353431200023PubMedID: 25422108OAI: diva2:798433
Available from: 2015-03-26 Created: 2015-03-26 Last updated: 2016-03-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ludvigsson, Johnny
By organisation
Division of Clinical SciencesFaculty of Health SciencesDepartment of Paediatrics in Linköping
In the same journal
Infectious Medicine

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 182 hits
ReferencesLink to record
Permanent link

Direct link