Sciatic nerve blood flow measured by laser Doppler flowmetry and [14C]iodoantipyrine.
1985 (English)In: American Journal of Physiology, ISSN 0002-9513, Vol. 248, no 3 Pt 2, H311-317 p.Article in journal (Refereed) Published
Blood flow was examined in sciatic nerves of pentobarbital-anesthetized rats by means of laser Doppler flowmetry (LDF) and intravenous [14C]iodoantipyrine infusion. Continuous LDF signals demonstrated slow oscillations and acute, pressure-related changes in flow. The steady-state LDF signal was related linearly to nerve blood flow, as measured with [14C]iodoantipyrine, in intact nerves and nerves stripped of the epineurium. In 14 intact nerves, nerve blood flow averaged 0.27 +/- 0.03 (SE) ml X min-1 X g-1, whereas it averaged 0.13 +/- 0.01 in 5 stripped nerves. Autoradiographs of [3H]-nicotine-infused nerves and intra-arterial injection of 57Co-labeled microspheres demonstrated that flow was not uniform throughout the nerve cross section. The results indicate that LDF can be used to examine nerve blood flow in vivo, demonstrate a linear relation between the LDF signal and flow, and establish absolute values for blood flow in intact and stripped nerves of the anesthetized rat.
Place, publisher, year, edition, pages
1985. Vol. 248, no 3 Pt 2, H311-317 p.
Fluid Mechanics and Acoustics
IdentifiersURN: urn:nbn:se:liu:diva-116885PubMedID: 3156511OAI: oai:DiVA.org:liu-116885DiVA: diva2:801307