Soft sensor application on lactate controlled fed-batch cultivation for monoclonal antibody production
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Monoclonal antibody producing cells are of great interest and used frequently in the field of biomedical research, diagnostics and therapy with increasing need for better systems to more efficiently produce antibodies at a lower costs. In this project three fed-batch cultivations of hybridoma cells (HB-8696) were cultured in a stirred tank reactor with the use of a soft sensor to monitor the lactate concentration and as well as a dielectric probe for biomass measurements. In addition, a protocol for growing the inoculum was also successfully produced and a previous batch cultivation was also analyzed which gave crucial information about stoichiometrically relation in the feed medium which was used in the fed-batch cultivations. The BioSenz Analyzer was used for on-line lactate concentration monitoring and was later used to control the feed profile to avoid overflow metabolism in two of the three fed-batch cultivations. However, nothing conclusive could be said about the lactate controller as of yet which needs further research.
Place, publisher, year, edition, pages
2015. , 29 p.
IdentifiersURN: urn:nbn:se:liu:diva-117179ISRN: LITH-IFM-A-EX--15/2983--SEOAI: oai:DiVA.org:liu-117179DiVA: diva2:806700
Subject / course