The activity of paraoxonase type 1 (PON-1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility
2015 (English)In: Andrology, ISSN 2047-2919, E-ISSN 2047-2927, Vol. 3, no 2, 315-320 p.Article in journal (Refereed) Published
Paraoxonase 1 (PON-1) is a hydrolytic enzyme present in body fluids, capable of protecting cells against oxidative stress. The hypothesis was hereby to test that PON-1, present in seminal plasma (SP), acts protecting boar spermatozoa when showing a reasonable high activity in the ejaculate. SP-PON-1 activity differed (pless than0.001) among boars (from 0.10 to 0.29IU/mL). Intra-boar variability was also observed (pless than0.05), but only in two of the 15 boars. SP-PON-1 activity differed among ejaculate portions, showing the spermatozoa-peak portion of spermatozoa-rich ejaculate fraction the highest levels (0.35 +/- 0.03IU/mL, ranging from 0.12 to 0.69) and the post-sperm ejaculate fraction the lowest levels (0.12 +/- 0.01IU/mL, ranging from 0.03 to 0.21). SP-PON-1 activity was positively correlated with the percentage of spermatozoa with rapid and progressive movement (pless than0.01) and negatively correlated with the generation of intracellular reactive oxygen species (pless than0.01) in semen samples after 72h of liquid storage. SP-PON-1 activity was highest (pless than0.01) in boars with highest farrowing rates. In conclusion, SP-PON-1 activity differed among boars and ejaculate fractions/portions. SP-PON-1 activity was positively correlated with sperm quality and functionality of liquid-stored semen samples and it evidenced a positive association with in vivo fertility.
Place, publisher, year, edition, pages
American Society of Andrology , 2015. Vol. 3, no 2, 315-320 p.
boar; paraoxonase type 1; semen storage; seminal plasma; sperm functionality; sperm quality
IdentifiersURN: urn:nbn:se:liu:diva-118066DOI: 10.1111/andr.309ISI: 000353398800023PubMedID: 25598515OAI: oai:DiVA.org:liu-118066DiVA: diva2:812865
Funding Agencies|MINECO Madrid (Spain) [AGL2012-39903]; FEDER funds (EU); MECD (Madrid, Spain); Seneca Foundation (Murcia, Spain); Science Without Borders Program (Brazilian Scientific Mobility Program at CNPq/MCT)2015-05-202015-05-202016-04-25