Determination of carnitine and acylcarnitines in human urine by means of microextraction in packed sorbent and hydrophilic interaction chromatography-ultra-high-performance liquid chromatography-tandem mass spectrometry
2015 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 109, 171-176 p.Article in journal (Refereed) Published
A method using semi-automatic microextraction by packed sorbent (eVol (R)-MEPS) and hydrophilic interaction chromatography-ultra-high-performance liquid chromatography-tandem mass spectrometry (HILIC-UHPLC-MS/MS) was described for the simultaneous determination of carnitine and acylcarnitines in human urine. The optimal conditions of MEPS extraction were obtained using C2 of M1 (C8 + SCX) phase as a sorbent Chromatographic separation of the analytes was achieved within 2.5 min on Acquity UPLC BEH HILIC column using a gradient elution program with water containing 5 mM ammonium acetate and acetonitrile as the mobile phase. The detection was performed on a triple-quadrupole tandem mass spectrometer in a positive ion mode via electrospray ionization (ESI). The linearity of the calibration curves for all compounds was found over a range from 0.1 ng/mL to 500 ng/mL. The method afforded satisfactory results in terms of sensitivity, specificity, precision, accuracy, recovery as well as stability of the analyte under various conditions. The method was used successfully for determination of carnitine and acylcarnitines in human urine.
Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 109, 171-176 p.
Carnitine; Acylcarnitines; HILIC; Microextraction
IdentifiersURN: urn:nbn:se:liu:diva-118027DOI: 10.1016/j.jpba.2015.02.044ISI: 000353078700023PubMedID: 25770415OAI: oai:DiVA.org:liu-118027DiVA: diva2:813109
Funding Agencies|National Science Centre (Cracow, Poland) [N N204 355840]2015-05-212015-05-202015-05-28