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Label free impedimetric Salmonella aptasensor based on pyrrole (pyrrole -3-carboxyl acid ) copolymer.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-2071-7768
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
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2015 (English)In: Sweden-Japan Seminar on Nanomaterials and Nanotechnology – SJS-Nano, Linköping, Sweden, 10-11 March 2015, Japan Society for the Promotion of Science (JSPS), Stockholm. , 2015, 32-33 p.Conference paper, Poster (Refereed)
Abstract [en]

Salmonella is a Gram-negative foodborne pathogen that can cause gastrointestinal infection that is the cause of numerous hospitalisations and deaths all over the world [1]. Conventional approaches for Salmonella detection, based on culture methods, are time-consuming and labour-intensive; this creates considerable need for the development of novel, fast and reliable approaches.

Conductive polymers are poly-conjugated systems that present, at the same time, the properties of conductive materials and conventional polymers. Among them, polypyrrole and its derivatives are attracting a lot of attention in several fields including actuators and biosensors [2].

 

Aptamers are single strand of DNA or RNA that can bind to specific target with high affinity showing in this way great potentiality as alternative to antibodies in affinity based biosensors [3].

 

In the study presented herein the preparation, via electrodeposition, of a copolymer based on pyrrole and pyrrol 3-carboxylic acid and its application in the development of an aptamer based biosensor is presented. Immobilisation of aptamers, via EDC /NHS chemistry onto the synthetised polymer has been demonstrated via electrochemical techniques. The detection of different concentration of Salmonella was performed by incubation of the prepared electrode with different concentrations of bacteria, followed by impedance measurement in LiClO4 solution. A Nyquist plot of impedance spectra showed increase in the radii of the semicircle, corresponding to an increased charge transfer resistance, and associated to the interaction between the immobilised aptames and the bacteria in the sample. This initial result suggests that it should be possible to create a label-free sensor based on this method.

 

[1] J. Yuan, Z. Tao, Y.Yu, X. Ma, Y. Xia, L. Wang, Z. Wang,Food Control 37 (2014) 188 – 192

[2] R. Balint, N. J. Cassidy, S. H. Cartmell, Acta Biomaterialia 10 (2014) 2341–2353

[3] S. Tombelli, M. Minunni, M. Mascini, Biosensors and Bioelectronics 20 (2005) 2424–2434

Place, publisher, year, edition, pages
Japan Society for the Promotion of Science (JSPS), Stockholm. , 2015. 32-33 p.
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:liu:diva-118100OAI: oai:DiVA.org:liu-118100DiVA: diva2:813172
Conference
Sweden-Japan Seminar on Nanomaterials and Nanotechnology – SJS-Nano, Linköping, Sweden, 10-11 March 2015
Available from: 2015-05-21 Created: 2015-05-21 Last updated: 2016-05-24

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Jager, EdwinGolabi, MohsenTurner, AnthonyBeni, Valerio
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