Tunable Conjugated Polymers for Bacterial Differentiation
2015 (English)In: 4th International Conference on Bio-Sensing Technology, 10-13 May 2015, Lisbon, Portugal., Elsevier, 2015Conference paper, Abstract (Refereed)
A novel rapid method for bacterial differentiation is explored based on the specific adhesion pattern of bacteria to tunable polymer surfaces. Different types of counter ions were used to electrochemically fabricate dissimilar polypyrrole (PPy) films with diverse physicochemical properties such as hydrophobicity, thickness and roughness. In order to expand the number of individual sensors in the array, three different redox states (as fabricated, oxidised and reduced) of each PPy film were also employed. These dissimilar PPy surfaces were exposed to five different bacteria, Deinococcus proteolyticus, Staphylococcus epidermidis, Alcaligenes faecalis, Pseudomonas fluorescens and Serratia marcescens, , which were seeded onto the various PPy surfaces. Fluorescent microscope images were taken and used to quantify the number of cells adhering to the surfaces. Generally, the number of cells of a particular bacterial strain that adhered varied when exposed to dissimilar polymer surfaces, due to the effects of the surface properties of the polymer on bacterial attachment. Similarly, the number of cells that adhered varied with different bacteria exposed to the same surface, reflecting the different surface properties of the bacteria. Statistical analysis and principal component analysis showed that all had their own specific adhesion pattern with respect to the array of PPy surfaces. Hence, these bacteria could be discriminated by this simple label-free method. In summary, this provides a proof-of-concept for using specific adhesion properties of bacterial in conjunction with tunable polymer arrays and pattern recognition as a method for rapid bacterial identification in situ.
Place, publisher, year, edition, pages
Textile, Rubber and Polymeric Materials
IdentifiersURN: urn:nbn:se:liu:diva-118107OAI: oai:DiVA.org:liu-118107DiVA: diva2:813191
4th International Conference on Bio-Sensing Technology, 10-13 May 2015, Lisbon, Portugal.