Holographic microscopy provides new insights into the settlement of zoospores of the green alga Ulva linza on cationic oligopeptide surfaces
2015 (English)In: Biofouling (Print), ISSN 0892-7014, E-ISSN 1029-2454, Vol. 31, no 2, 229-239 p.Article in journal (Refereed) Published
Interaction of zoospores of Ulva linza with cationic, arginine-rich oligopeptide self-assembled monolayers (SAMs) is characterized by rapid settlement. Some spores settle (ie permanently attach) in a normal manner involving the secretion of a permanent adhesive, retraction of the flagella and cell wall formation, whilst others undergo pseudosettlement whereby motile spores are trapped (attached) on the SAM surface without undergoing the normal metamorphosis into a settled spore. Holographic microscopy was used to record videos of swimming zoospores in the vicinity of surfaces with different cationic oligopeptide concentrations to provide time-resolved insights into processes associated with attachment of spores. The data reveal that spore attachment rate increases with increasing cationic peptide content. Accordingly, the decrease in swimming activity in the volume of seawater above the surface accelerated with increasing surface charge. Three-dimensional trajectories of individual swimming spores showed a hit and stick motion pattern, exclusively observed for the arginine-rich peptide SAMs, whereby spores were immediately trapped upon contact with the surface.
Place, publisher, year, edition, pages
Taylor & Francis: STM, Behavioural Science and Public Health Titles , 2015. Vol. 31, no 2, 229-239 p.
holographic microscopy; cationic peptides; zoospores; Ulva linza; tracking; 3-dimensional motility
IdentifiersURN: urn:nbn:se:liu:diva-118256DOI: 10.1080/08927014.2015.1022534ISI: 000353565900010PubMedID: 25875964OAI: oai:DiVA.org:liu-118256DiVA: diva2:813528
Funding Agencies|US Office of Naval Research [N000141210498, N0001-08-1-0010]; EU project AMBIO; DFG [RO 2524/2-2]2015-05-222015-05-222015-05-22