liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Capacitive biosensor for detection of toxicity biomarkers
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
2015 (English)Manuscript (preprint) (Other academic)
Abstract [en]

Microfluidic devices are rapidly gaining importance for in vitro toxicity testing. Biomarker detection in microfluidic assays are however challenging due to small sample sizes and low analyte concentration. Capacitive electrochemical biosensors have been reported to have high sensitivity and properties that are amenable for implementation into microfluidic devices.

In this work a biosensor application for troponin T (TnT) is presented. The sensor showed linear response to analyte over five orders of magnitude with the lowest detectable signal at 10-13 M. The sensor proved to be able to detect TnT spiked in cell culture media at concentrations relevant for cell cultures.

Place, publisher, year, edition, pages
2015.
National Category
Biological Sciences Physical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-118293OAI: oai:DiVA.org:liu-118293DiVA: diva2:814064
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2015-05-26
In thesis
1. Microfluidic biosensor systems for cardiotoxicity assaying
Open this publication in new window or tab >>Microfluidic biosensor systems for cardiotoxicity assaying
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Toxicity screening is an important part of pharmaceutical development and early detection of toxic side effects provide the opportunity for early redesign or termination of unfeasible projects. Today toxicity testing is relying on experiments on animals. Ethical concerns, high costs and problems with interspecies variability in animal experiments have introduced incentives for cell-based toxicity assays. The recent development of stem cell technology have raised the hope for toxicity testing with higher predictivity that can reduce the amount of animals sacrificed, increase the patient safety and reduce the costs in pharmaceutical development.

Cell development and behavior is to a large extent dependent on the microenvironment. Microfluidic techniques can be used to build small-sized structures that provide the opportunity to introduce a high degree of control of the cell culture environment with features in cell sizes. In this thesis is demonstrated two different methods for infusing cells into microfluidic cell culture devices using either cells clustered in cardiac bodies during differentiation or cells pre-seeded in microporous carriers prior to infusion.

Microfluidic cell culture devices are well suited for optical  evaluation. Demonstrated in this thesis is fluorescent staining in combination with confocal microscopy as well as automated imaging with evaluation of beating frequency of cardiomyocyte cell clusters can be used to assess toxicity of cells cultured in microfluidic devices.

Biosensors use biological recognition elements to measure the presence of a chemical substance, for example low concentrations of biomarkers secreted by cells in a toxicity assay. Especially capacitive biosensors have shown very low limit of detection. In addition, protein G is demonstrated as an affinity ligand to capture IgG antibodies used as recognition element in a biosensor application or used for antibody screening.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2015. 49 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1678
National Category
Biological Sciences Physical Sciences
Identifiers
urn:nbn:se:liu:diva-118295 (URN)978-91-7519-046-4 (print) (ISBN)
Public defence
2015-06-12, Visionen, B-huset, Campus Valla, Linköping, 14:00 (Swedish)
Opponent
Supervisors
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2015-10-02Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Bergström, GunnarKuusk, AveMandenius, Carl-Fredrik
By organisation
BiotechnologyThe Institute of TechnologyDepartment of Physics, Chemistry and BiologyFaculty of Science & Engineering
Biological SciencesPhysical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 244 hits
ReferencesLink to record
Permanent link

Direct link