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Control of hydrogen peroxide and glucose via UV and Visible Photoluminescence of ZnO nanoparticles.
Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus, Belarus .
Linköping University, Department of Physics, Chemistry and Biology, Semiconductor Materials. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0001-6889-0351
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-1815-9699
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2015 (English)In: Microchimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 182, no 9-10, 1819-1826 p.Article in journal (Refereed) Published
Abstract [en]

We report on an indirect optical method for the determination of glucose via the detection of hydrogen peroxide (H2O2) that is generated during the glucose oxidase (GOx) catalyzed oxidation of glucose. It is based on the finding that the ultraviolet (~374 nm) and visible (~525 nm) photoluminescence of pristine zinc oxide (ZnO) nanoparticles strongly depends on the concentration of H2O2 in water solution. Photoluminescence is quenched by up to 90 % at a 100 mM level of H2O2. The sensor constructed by immobilizing GOx on ZnO nanoparticles enabled glucose to be continuously monitored in the 10 mM to 130 mM concentration range, and the limit of detection is 10 mM. This enzymatic sensing scheme is supposed to be applicable to monitoring glucose in the food, beverage and fermentation industries. It has a wide scope in that it may be extended to numerous other substrate or enzyme activity assays based on the formation of H2O2, and of assays based on the consumption of H2O2 by peroxidases.

Place, publisher, year, edition, pages
Springer Netherlands, 2015. Vol. 182, no 9-10, 1819-1826 p.
National Category
Materials Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-118305DOI: 10.1007/s00604-015-1493-9ISI: 000356449700030OAI: oai:DiVA.org:liu-118305DiVA: diva2:814288
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2015-07-13

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Khranovskyy, VolodymyrBeni, ValerioTurner, Anthony P FEriksson, Martin OHoltz, Per-OlofYakimova, Rositsa
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Semiconductor MaterialsFaculty of Science & EngineeringBiosensors and Bioelectronics
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Microchimica Acta
Materials Chemistry

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