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Surface functionalisation of carbon for low cost fabrication of highly stable electrochemical DNA sensors
Universitat Rovira i Virgili.
Universitat Rovira i Virgili.
Departament d'Enginyeria Química, Universitat Rovira i Virgili, Avinguda Països Catalans, 26, 43007 Tarragona, Spain.ORCID iD: 0000-0001-6889-0351
Universitat Rovira i Virgili.
2015 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 71, 25-29 p.Article in journal (Refereed) Published
Abstract [en]

An alternative strategy for surface tethering of DNA probes, where highly reactive glassy carbon (GC) substrates are prepared via electrochemical hydrogenation and electrochemical/chemical chlorination is reported. Thiolated DNA probes and alkanethiols were stably immobilised on the halogenated carbon, with electrochemical chlorination being milder, thus producing less damage to the surface. Electrochemical DNA sensors prepared using this surface  chemistry on carbon with electrochemical chlorination providing an improved performance,producing a highly ordered surface and the use of lateral spacers to improve steric  accessibility to immobilised probes was not required.

Place, publisher, year, edition, pages
2015. Vol. 71, 25-29 p.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-118306DOI: 10.1016/j.bios.2015.03.076OAI: oai:DiVA.org:liu-118306DiVA: diva2:814291
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2017-10-17

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Beni, Valerio
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