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DNA biosensor based on hybridization refractory mutation system approach for single mismatch detection
Universitat Rovira i Virgili, 43007 Tarragona, Spain.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. INTERFIBIO Research Group, Departament d’Enginyeria Quimica, Universitat Rovira i Virgili, 43007 Tarragona, Spain.ORCID iD: 0000-0001-6889-0351
Universitat Rovira i Virgili, 43007 Tarragona, Spain. (Universitat Rovira i Virgili)
Universitat Rovira i Virgili, 43007 Tarragona, Spain.
2015 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 474, 66-68 p.Article in journal (Refereed) Published
Abstract [en]

We report on a simple approach to enhance solid-phase hybridization-based single base mismatch discrimination at high ionic strength based on the deliberate insertion of a natural DNA base mismatch in the surface-tethered probe. A large drop in hybridization signal of single base mismatched alleles using the designed probe as compared with the conventional probe, from 80% to less than 25% of the signal obtained with the fully complementary, non-mutation-containing sequence, when using colorimetric detection was further improved to 20% when using electrochemical detection, attributable to a difference of spacing of immobilized probes. Finally, the designed probe was used for the electrochemical detection of the DQA1*05:05 allele amplified from real human blood samples.

Place, publisher, year, edition, pages
2015. Vol. 474, 66-68 p.
National Category
Genetics Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-118307DOI: 10.1016/j.ab.2014.11.021PubMedID: 25524618OAI: oai:DiVA.org:liu-118307DiVA: diva2:814295
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2017-10-17

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Beni, Valerio
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