DNA biosensor based on hybridization refractory mutation system approach for single mismatch detection
2015 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 474, 66-68 p.Article in journal (Refereed) Published
We report on a simple approach to enhance solid-phase hybridization-based single base mismatch discrimination at high ionic strength based on the deliberate insertion of a natural DNA base mismatch in the surface-tethered probe. A large drop in hybridization signal of single base mismatched alleles using the designed probe as compared with the conventional probe, from 80% to less than 25% of the signal obtained with the fully complementary, non-mutation-containing sequence, when using colorimetric detection was further improved to 20% when using electrochemical detection, attributable to a difference of spacing of immobilized probes. Finally, the designed probe was used for the electrochemical detection of the DQA1*05:05 allele amplified from real human blood samples.
Place, publisher, year, edition, pages
2015. Vol. 474, 66-68 p.
Genetics Analytical Chemistry
IdentifiersURN: urn:nbn:se:liu:diva-118307DOI: 10.1016/j.ab.2014.11.021PubMedID: 25524618OAI: oai:DiVA.org:liu-118307DiVA: diva2:814295