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Stability of gamma-Hydroxybutyrate in Blood Samples from Impaired Drivers after Storage at 4A degrees C and Comparison of GC-FID-GBL and LC-MS-MS Methods of Analysis
Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Medicine and Health Sciences. National Board Forens Med, Department Forens Genet and Forens Toxicol, Linkoping, Sweden.
National Board Forens Med, Department Forens Genet and Forens Toxicol, Linkoping, Sweden.
University of Copenhagen, Denmark.
University of Copenhagen, Denmark.
2015 (English)In: Journal of Analytical Toxicology, ISSN 0146-4760, Vol. 39, no 4, 294-299 p.Article in journal (Refereed) Published
Abstract [en]

The stability of gamma-hydroxybutyrate (GHB) was determined in 50 blood samples from impaired drivers after storage at 4A degrees C for up to 12 months. GHB was determined in whole blood by gas chromatography-flame ionization detector (GC-FID) after conversion into gamma-butyrolactone (GBL) and results were compared with LC-MS-MS. Both analytical methods showed a linear response (R-2 greater than 0.99) to GHB concentrations from 2 to 250 mg/kg. The mean decrease in concentration after storage was 4.8 mg/kg, with extreme changes of +13 mg/kg or -29 mg/kg. Results by the GC-FID-GBL method (y-variate) and the LC-MS-MS method (x-variate) were highly correlated (R-2 = 0.974). The regression equation was y = 0.85x + 2.2 and residual standard deviation (SD) was 7.8 mg/kg. The y-intercept (2.2 mg/kg) was not significantly different from zero (P greater than 0.05), although the slope of the regression line (0.85) differed from unity (P less than 0.001), indicating a proportional bias of 15%. The LC-MS-MS method tended to give higher results than the GC-FID-GBL method. The mean difference (bias) was 12 mg/kg (P less than 0.001). The SD of individual differences was 11.3 mg/kg and 95% limits of agreement were -11 to +33 mg/kg. The results of this study show that concentrations of GHB in whole blood are stable during storage at 4A degrees C for up to 6 months.

Place, publisher, year, edition, pages
Oxford University Press (OUP): Policy F , 2015. Vol. 39, no 4, 294-299 p.
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Clinical Medicine
Identifiers
URN: urn:nbn:se:liu:diva-119258DOI: 10.1093/jat/bkv007ISI: 000354695800006PubMedID: 25652881OAI: oai:DiVA.org:liu-119258DiVA: diva2:820726
Available from: 2015-06-12 Created: 2015-06-12 Last updated: 2015-06-12

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Jones, A Wayne
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