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Simultaneous determination of 40 novel psychoactive stimulants in urine by liquid chromatography-high resolution mass spectrometry and library matching
NIDA, MD 21224 USA.
NIDA, MD 21224 USA; University of Maryland Baltimore County, MD 21228 USA.
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Medicine and Health Sciences. National Board Forens Med, Department Forens Genet and Forens Toxicol, Linkoping, Sweden.
NIDA, MD 21224 USA.
2015 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1397, 32-42 p.Article in journal (Refereed) Published
Abstract [en]

The emergence of novel psychoactive substances is an ongoing challenge for analytical toxicologists. Different analogs are continuously introduced in the market to circumvent legislation and to enhance their pharmacological activity. Although detection of drugs in blood indicates recent exposure and link intoxication to the causative agent, urine is still the most preferred testing matrix in clinical and forensic settings. We developed a method for the simultaneous quantification of 8 piperazines, 4 designer amphetamines and 28 synthetic cathinones and 4 metabolites, in urine by liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Data were acquired in full scan and data dependent MS2 mode. Compounds were quantified by precursor ion exact mass, and confirmed by product ion spectra library matching, taking into account product ions exact mass and intensities. One-hundred pi, urine was subjected to solid phase cation exchange extraction (SOLA SCX). The chromatographic reverse-phase separation was achieved with gradient mobile phase of 0.1% formic acid in water and in acetonitrile in 20 min. The assay was linear from 2.5 or 5 to 500 mu g/L. Imprecision (n = 15) was less than15.4%, and accuracy (n = 15) 84.2-118.5%. Extraction efficiency was 51.2-111.2%, process efficiency 57.7-104.9% and matrix effect ranged from -41.9% to 238.5% (CV less than 23.3%, except MDBZP CV less than 34%). Authentic urine specimens (n = 62) were analyzed with the method that provides a comprehensive confirmation for 40 new stimulant drugs with specificity and sensitivity.

Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 1397, 32-42 p.
Keyword [en]
LC-HRMS; Novel psychoactive substance; Synthetic cathinones; Piperazines; Amphetamines; Urine
National Category
Analytical Chemistry
URN: urn:nbn:se:liu:diva-119231DOI: 10.1016/j.chroma.2015.04.002ISI: 000355029300005PubMedID: 25931378OAI: diva2:821292

Funding Agencies|Intramural Research Program of the National Institute on Drug Abuse, National Institutes of Health

Available from: 2015-06-15 Created: 2015-06-12 Last updated: 2015-07-02

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Kronstrand, Robert
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