Molecular characterization and genetic susceptibility of sapovirus in children with diarrhea in Burkina Faso
2015 (English)In: Infection, Genetics and Evolution, ISSN 1567-1348, E-ISSN 1567-7257, Vol. 32, 396-400 p.Article in journal (Refereed) Published
Sapoviruses (Says) are a common cause of gastroenteritis in children. In sub-Saharan Africa, there is a scarcity of information regarding SaV as an etiological agent of diarrhea. Here, we investigated the prevalence, molecular characterization and clinico-epidemiological features of SaV infections in children less than 5 years of age with diarrhea in Burkina Faso. We further investigated the role of type 1 histo blood group antigens as susceptibility factors. In total, 309 fecal and 208 saliva samples from diarrheal children in Ouagadougou, Burkina Faso, were collected between May 2009 and March 2010. Say was detected using real-time PCR, and genogrouped/genotyped by PCR or sequencing. Saliva samples were ABO, Lewis and secretor phenotyped using in house ELISA assays. We found a high prevalence (18%) and large genetic diversity with all 4 human genogroups, and 9 genotypes/genoclusters circulating during the study period. The SaV infections were generally associated with milder symptoms, and neither ABH, Lewis or secretor phenotypes affected susceptibility to Say infections. (C) 2015 Published by Elsevier B.V.
Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 32, 396-400 p.
Sapovirus; Burkina Faso; Gastroenteritis; Susceptibility; Histo-blood group antigens
IdentifiersURN: urn:nbn:se:liu:diva-119575DOI: 10.1016/j.meegid.2015.03.039ISI: 000355027300051PubMedID: 25847694OAI: oai:DiVA.org:liu-119575DiVA: diva2:825321
Funding Agencies|Swedish Research Council 2015-06-232015-06-222015-06-23