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Monitoring the dynamics of syntrophic beta-oxidizing bacteria during anaerobic degradation of oleic acid by quantitative PCR
University of Washington, WA 98195 USA.
University of Washington, WA 98195 USA; University of Washington, WA 98195 USA.
Linköping University, The Tema Institute, Tema Environmental Change. Linköping University, Faculty of Arts and Sciences.ORCID iD: 0000-0002-1927-4656
University of Washington, WA 98195 USA.
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2015 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 91, no 4Article in journal (Refereed) Published
Abstract [en]

The ecophysiology of long-chain fatty acid-degrading syntrophic beta-oxidizing bacteria has been poorly understood due to a lack of quantitative abundance data. Here, TaqMan quantitative PCR (qPCR) assays targeting the 16S rRNA gene of the known mesophilic syntrophic beta-oxidizing bacterial genera Syntrophomonas and Syntrophus were developed and validated. Microbial community dynamics were followed using qPCR and Illumina-based high-throughput amplicon sequencing in triplicate methanogenic bioreactors subjected to five consecutive batch feedings of oleic acid. With repeated oleic acid feeding, the initial specific methane production rate significantly increased along with the relative abundances of Syntrophomonas and methanogenic archaea in the bioreactor communities. The novel qPCR assays showed that Syntrophomonas increased from 7 to 31% of the bacterial community 16S rRNA gene concentration, whereas that of Syntrophus decreased from 0.02 to less than 0.005%. High-throughput amplicon sequencing also revealed that Syntrophomonas became the dominant genus within the bioreactor microbiomes. These results suggest that increased specific mineralization rates of oleic acid were attributed to quantitative shifts within the microbial communities toward higher abundances of syntrophic beta-oxidizing bacteria and methanogenic archaea. The novel qPCR assays targeting syntrophic beta-oxidizing bacteria may thus serve as monitoring tools to indicate the fatty acid beta-oxidization potential of anaerobic digester communities.

Place, publisher, year, edition, pages
Oxford University Press (OUP): Policy B - Oxford Open Option D , 2015. Vol. 91, no 4
Keyword [en]
syntrophy; beta-oxidization; anaerobic digestion; quantitative PCR (qPCR); high-throughput sequencing; Syntrophomonas; Syntrophus; methanogenesis; long-chain fatty acids (LCFA)
National Category
Microbiology
Identifiers
URN: urn:nbn:se:liu:diva-119810DOI: 10.1093/femsec/fiv028ISI: 000355327300011PubMedID: 25873606OAI: oai:DiVA.org:liu-119810DiVA: diva2:827162
Note

Funding Agencies|Biogas Research Center at Linkoping University; US National Science Foundation Graduate Research Fellowship [DGE-1256082]; University of Washington Valle Scandinavian Exchange Fellowship

Available from: 2015-06-26 Created: 2015-06-26 Last updated: 2015-08-13

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Genero, Magalí MartíSvensson, Bo
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