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Bone Alkaline Phosphatase Isoforms in Hemodialysis Patients With Low Versus Non-Low Bone Turnover: A Diagnostic Test Study
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Heart and Medicine Center, Department of Nephrology. Karolinska University Hospital, Sweden.
University of Kentucky, KY 40536 USA.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Chemistry.
University of Kentucky, KY 40536 USA.
2015 (English)In: American Journal of Kidney Diseases, ISSN 0272-6386, E-ISSN 1523-6838, Vol. 66, no 1, 99-105 p.Article in journal (Refereed) Published
Abstract [en]

Background: Renal osteodystrophy encompasses the bone histologic abnormalities seen in patients with chronic kidney disease (CKD). The bone-specific alkaline phosphatase (bALP) isoform B1x is exclusively found in serum of some patients with CKD. Study Design: The aim of this cross-sectional diagnostic test study was to examine the relationship between serum bALP isoform activity and histomorphometric parameters of bone in patients with CKD receiving maintenance hemodialysis. Settings and Participants: Anterior iliac crest bone biopsy samples from 40 patients with CKD were selected on the basis of bone turnover for histomorphometric analysis. There were samples from 20 patients with low and 20 with non-low bone turnover. Index Test: In serum, bALP, bALP isoforms (B/I, B1x, B1, and B2), and parathyroid hormone (PTH) were measured. Reference Test: Low bone turnover was defined by mineral apposition rate, 0.36 mu m/d. Non-low bone turnover was defined by mineral apposition rate greater than= 0.36 mu m/d. Other Measurements: PTH. Results: B1x was found in 21 patients (53%) who had lower median levels of bALP, 18.6 versus 46.9 U/L; B/I, 0.10 versus 0.22 mu kat/L; B1, 0.40 versus 0.88 mu kat/L; B2, 1.21 versus 2.66 mu kat/L; and PTH, 49 versus 287 pg/mL, compared with patients without B1x (P less than 0.001). 13 patients (65%) with low bone turnover and 8 patients (40%) with non-low bone turnover (P less than 0.2) had detectable B1x. B1x correlated inversely with histomorphometric parameters of bone turnover. Receiver operating characteristic curves showed that B1x can be used for the diagnosis of low bone turnover (area under the curve [AUC], 0.83), whereas bALP (AUC, 0.89) and PTH (AUC, 0.85) are useful for the diagnosis of non-low bone turnover. Limitations: Small number of study participants. Requirement of high-performance liquid chromatography methods for measurement of B1x. Conclusions: B1x, PTH, and bALP have similar diagnostic accuracy in distinguishing low from non-low bone turnover. The presence of B1x is diagnostic of low bone turnover, whereas elevated bALP and PTH levels are useful for the diagnosis of non-low bone turnover.

Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 66, no 1, 99-105 p.
Keyword [en]
Bone-specific alkaline phosphatase (bALP); bALP isoforms; B/I, B1x, B1, B2; renal osteodystrophy; bone histology; low bone turnover; mineral apposition rate; parathyroid hormone (PTH); hemodialysis; end-stage renal disease (ESRD); diagnostic test study
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:liu:diva-120136DOI: 10.1053/j.ajkd.2015.02.323ISI: 000356730600019PubMedID: 25843703OAI: oai:DiVA.org:liu-120136DiVA: diva2:841644
Note

Funding Agencies|National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health (NIH) [R01 080770]; Kentucky Nephrology Research Trust; County Council of Ostergotland, Sweden

Available from: 2015-07-14 Created: 2015-07-13 Last updated: 2016-03-11

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Haarhaus, MathiasMagnusson, Per
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Division of Microbiology and Molecular MedicineFaculty of Medicine and Health SciencesDepartment of NephrologyDepartment of Clinical Chemistry
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