Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity
2015 (English)In: Toxicology and Applied Pharmacology, ISSN 0041-008X, E-ISSN 1096-0333, Vol. 287, no 1Article in journal (Refereed) Published
Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system.
Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 287, no 1
Microcystin-LR; microRNA; mRNA; Sertoli cell; Bioinformatics; Toxicity
IdentifiersURN: urn:nbn:se:liu:diva-120328DOI: 10.1016/j.taap.2015.05.008ISI: 000357766400001PubMedID: 25986756OAI: oai:DiVA.org:liu-120328DiVA: diva2:843915
Funding Agencies|National Natural Science Foundation of China [31370524, 31200401, 21377052, 81170054]; Natural Science Foundation of Jiangsu Province of China [BK2012307, BK20131281, BK2011570]; Specialized Research Fund for Doctoral Program of Higher Education (SRFDP) of Peoples Republic of China ; Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University [G5]2015-07-312015-07-312015-07-31