Vascular toxicity of ultra-small TiO2 nanoparticles and single walled carbon nanotubes in vitro and in vivo
2015 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 63Article in journal (Refereed) Published
Ultra-small nanoparticles (USNPs) at 1-3 nm are a subset of nanoparticles (NPs) that exhibit intermediate physicochemical properties between molecular dispersions and larger NPs. Despite interest in their utilization in applications such as theranostics, limited data about their toxicity exist. Here the effect of TiO2-USNPs on endothelial cells in vitro, and zebrafish embryos in vivo, was studied and compared to larger TiO2-NPs (30 nm) and to single walled carbon nanotubes (SWCNTs). In vitro exposure showed that TiO2-USNPs were neither cytotoxic, nor had oxidative ability, nevertheless were genotoxic. In vivo experiment in early developing zebrafish embryos in water at high concentrations of TiO2-USNPs caused mortality possibly by acidifying the water and caused malformations in the form of pericardial edema when injected. Myo1C involved in glomerular development of zebrafish embryos was upregulated in embryos exposed to TiO2-USNPs. They also exhibited anti-angiogenic effects both in vitro and in vivo plus decreased nitric oxide concentration. The larger TiO2-NPs were genotoxic but not cytotoxic. SWCNTs were cytotoxic in vitro and had the highest oxidative ability. Neither of these NPs had significant effects in vivo. To our knowledge this is the first study evaluating the effects of TiO2-USNPs on vascular toxicity in vitro and in vivo and this strategy could unravel USNPs potential applications. (C) 2015 Elsevier Ltd. All rights reserved.
Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 63
Ultra-small nanoparticles; Zebrafish; Endothelium; Angiogenesis; Genotoxicity
IdentifiersURN: urn:nbn:se:liu:diva-120437DOI: 10.1016/j.biomaterials.2015.05.044ISI: 000357910400001PubMedID: 26066004OAI: oai:DiVA.org:liu-120437DiVA: diva2:845701
Funding Agencies|Swedish Research Council-Natural Science (VR-NT); Carl Trygger Foundation; VIN-NOVA-Vinnmer program; Oscar and Lilli Lamms Minne Foundation; IKERBASQUE, Basque Foundation for science; Basque Foundation for science; ALF; LiU; Angpanneforening Research foundation2015-08-122015-08-112016-04-24