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Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress
Islamic Azad University, Iran.
Islamic Azad University, Iran.
Islamic Azad University, Iran; Islamic Azad University, Iran.
University of Tehran, Iran.
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2015 (English)In: BMC Complementary and Alternative Medicine, ISSN 1472-6882, Vol. 15, no 246Article in journal (Refereed) Published
Abstract [en]

Background: Methotrexate (MTX) is an antimetabolite broadly used in treatment of cancer and autoimmune diseases. MTX-induced hepatotoxicity limits its application. We investigated hepatoprotective effects of turmeric in MTX-induced liver toxicity. Methods: All experiments were performed on male Wistar albino rats that were randomly divided into six groups. Group one received saline orally for 30 days (control group), groups two and three received turmeric extract (100, 200 mg/kg respectively) orally for 30 days, group four received single dose, of MTX IP at day 30, groups five and six received turmeric extract 100 and 200 mg/kg orally respectively for 30 days and single dose of methoterxate IP (20 mg/kg) at day 30. Four days after MTX injection animals were sacrificed and evaluated. Blood ALT and AST (indicators of hepatocyte injury), ALP and bilirubin (markers of biliary function), albumin (reflect liver synthetic function) as well as the plasma TAS concentration (antioxidant defenses) were determined. The cellular antioxidant defense activities were examined in liver tissue samples using SOD, CAT, and GSH-Px for the oxidative stress, and MDA for lipid peroxidation. In addition, liver damage was evaluated histopathologically. Results: MTX significantly induced liver damage (P less than 0.05) and decreased its antioxidant capacity, while turmeric was hepatoprotective. Liver tissue microscopic evaluation showed that MTX treatment induced severe centrilobular and periportal degeneration, hyperemia of portal vein, increased artery inflammatory cells infiltration and necrosis, while all of histopathological changes were attenuated by turmeric (200 mg/kg). Conclusion: Turmeric extract can successfully attenuate MTX-hepatotoxicity. The effect is partly mediated through extracts antinflammatory activity.

Place, publisher, year, edition, pages
BioMed Central , 2015. Vol. 15, no 246
Keyword [en]
Lipid peroxides; Total antioxidant status; Antioxidant enzymes; Serum liver biomarkers; Hepatocellular injury; Cell death
National Category
Clinical Medicine
URN: urn:nbn:se:liu:diva-120647DOI: 10.1186/s12906-015-0773-6ISI: 000358201500004PubMedID: 26199067OAI: diva2:847591

Funding Agencies|University of Manitoba start-up fund; Linkoping University; IGEN; Cancerfonden [2013/391]; VR-NanoVision [K2012-99X-22325-01-5]

Available from: 2015-08-20 Created: 2015-08-20 Last updated: 2015-08-25

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Los, Marek Jan
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Division of Cell BiologyFaculty of Medicine and Health Sciences
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