Collagen-Silver Nanowire Composites as Electrically Activeand Antibacterial Scaffolds for Embryonic Cardiac Cell Proliferation
(English)Manuscript (preprint) (Other academic)
Electroactive biomaterials are used in a number of applications, including scaffolds for neural and cardiac regeneration. Most electrodes and conductive scaffolds for tissue regeneration are based on synthetic materials that have limited biocompatibility and often display a large mismatch in mechanical properties with the surrounding tissue. In this work, we have developed a nanocomposite material prepared from self-assembled collagen and silver nanowires (AgNW) that display electrical properties analogous to electrodes used in the clinic. The AgNW concentration of the nanocomposites was optimized to stimulate proliferation of isolated embryonic cardiomyocytes. In addition, the AgNWs renders the nanocomposites antimicrobial against both Gram-negative Escherichia coli and Gram-positive Staphylococcus epidermidis. The mechanical properties of the nanocomposites were further characterized in physiological conditions and showed a dynamic modulus within the lower kPa range, suitable for embryonic cardiomyocyte proliferation. An in depth electrochemical analysis of the materials in the wet state showed a charge storage capacity of 12.28 mC cm-2, and charge injection capacity of 0.33 mC cm-2, comparable to electrode materials, such as iridium oxide and polypyrrole, currently used for electrical stimulation of tissues. The collagen/AgNW composites are thus multifunctional structural scaffolds that promote embryonic cardiomyocyte function, with the ability to store and inject charges, along with providing antimicrobial resistance.
Clinical Medicine Basic Medicine Physical Sciences
IdentifiersURN: urn:nbn:se:liu:diva-120770OAI: oai:DiVA.org:liu-120770DiVA: diva2:848294