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Tumor suppressive microRNA-137 negatively regulates Musashi-1 and colorectal cancer progression
University of Kansas, KS 66045 USA.
University of Kansas, KS 66045 USA.
University of Kansas, KS 66045 USA.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.
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2015 (English)In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 6, no 14, 12558-12573 p.Article in journal (Refereed) Published
Abstract [en]

Stem cell marker, Musashi-1 (MSI1) is over-expressed in many cancer types; however the molecular mechanisms involved in MSI1 over-expression are not well understood. We investigated the microRNA (miRNA) regulation of MSI1 and the implications this regulation plays in colorectal cancer. MicroRNA miR-137 was identified as a MSI1-targeting microRNA by immunoblotting and luciferase reporter assays. MSI1 protein was found to be highly expressed in 79% of primary rectal tumors (n=146), while miR-137 expression was decreased in 84% of the rectal tumor tissues (n=68) compared to paired normal mucosal samples. In addition to reduced MSI1 protein, exogenous expression of miR-137 inhibited cell growth, colony formation, and tumorsphere growth of colon cancer cells. Finally, in vivo studies demonstrated that induction of miR-137 can decrease growth of human colon cancer xenografts. Our results demonstrate that miR-137 acts as a tumor-suppressive miRNA in colorectal cancers and negatively regulates oncogenic MSI1.

Place, publisher, year, edition, pages
IMPACT JOURNALS LLC , 2015. Vol. 6, no 14, 12558-12573 p.
Keyword [en]
Tumor-initiating cells; microRNAs; RNA-binding proteins; colon cancer; rectal cancer
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:liu:diva-120884ISI: 000359008200060PubMedID: 25940441OAI: oai:DiVA.org:liu-120884DiVA: diva2:849361
Note

Funding Agencies|National Cancer Institute Cancer Center [P30_CA168524]

Available from: 2015-08-28 Created: 2015-08-28 Last updated: 2016-04-25

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Pathak, SurajitPing, JieSun, Xiao-Feng
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Division of Clinical SciencesFaculty of Medicine and Health SciencesDepartment of Oncology
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