Tumor suppressive microRNA-137 negatively regulates Musashi-1 and colorectal cancer progression
2015 (English)In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 6, no 14, 12558-12573 p.Article in journal (Refereed) Published
Stem cell marker, Musashi-1 (MSI1) is over-expressed in many cancer types; however the molecular mechanisms involved in MSI1 over-expression are not well understood. We investigated the microRNA (miRNA) regulation of MSI1 and the implications this regulation plays in colorectal cancer. MicroRNA miR-137 was identified as a MSI1-targeting microRNA by immunoblotting and luciferase reporter assays. MSI1 protein was found to be highly expressed in 79% of primary rectal tumors (n=146), while miR-137 expression was decreased in 84% of the rectal tumor tissues (n=68) compared to paired normal mucosal samples. In addition to reduced MSI1 protein, exogenous expression of miR-137 inhibited cell growth, colony formation, and tumorsphere growth of colon cancer cells. Finally, in vivo studies demonstrated that induction of miR-137 can decrease growth of human colon cancer xenografts. Our results demonstrate that miR-137 acts as a tumor-suppressive miRNA in colorectal cancers and negatively regulates oncogenic MSI1.
Place, publisher, year, edition, pages
IMPACT JOURNALS LLC , 2015. Vol. 6, no 14, 12558-12573 p.
Tumor-initiating cells; microRNAs; RNA-binding proteins; colon cancer; rectal cancer
Immunology in the medical area
IdentifiersURN: urn:nbn:se:liu:diva-120884ISI: 000359008200060PubMedID: 25940441OAI: oai:DiVA.org:liu-120884DiVA: diva2:849361
Funding Agencies|National Cancer Institute Cancer Center [P30_CA168524]2015-08-282015-08-282016-04-25